ABSTRACT

Introduction: Kidney dysfunction poses a high burden on patients and health care systems. Early detection and accurate prediction of kidney disease progression remains a major challenge. Compared to existing clinical parameters, urinary proteomics has the potential to reveal molecular alterations within the kidney that may alter its function before the onset of clinical symptoms. Thus, urinary proteomics has greater prognostic potential for assessment of kidney dysfunction progression.

Areas covered: Advances in urinary proteomics for major causes of kidney dysfunction are discussed. The application of urinary extracellular vesicles for studying kidney dysfunction are discussed. Technological advances in urinary proteomics are discussed. The literature was identified using a database search for titles containing ‘proteom*’ and ‘urin*’ and published within the past 5 years. Retrieved literature was manually filtered to retain kidney dysfunctions-related studies.

Expert opinion: Despite major advances, diagnosis by urinary proteomics has not been fully applied in any clinical settings. This could be attributed to the complex nature of kidney diseases, in addition to the constraints on study power and feasibility of incorporating mass spectrometry techniques in daily routine analysis. Nevertheless, we are confident that advances in urinary proteomics will soon provide superior insights into kidney disease beyond existing clinical parameters.

摘要

简介：肾功能不全给患者和医疗保健系统带来沉重负担。肾脏疾病进展的早期检测和准确预测仍然是一项重大挑战。与现有的临床参数相比，尿液蛋白质组学有可能揭示肾脏内的分子改变，这些改变可能会在临床症状出现之前改变其功能。因此，尿蛋白质组学在评估肾功能不全进展方面具有更大的预后潜力。

涵盖的领域：讨论了导致肾功能不全的主要原因的尿蛋白质组学进展。讨论了尿细胞外囊泡在肾功能障碍研究中的应用。讨论了尿蛋白质组学的技术进步。使用包含“蛋白质组*”和“尿素*”的标题的数据库搜索来确定文献，并在过去 5 年内发表。手动过滤检索到的文献以保留与肾功能障碍相关的研究。

专家意见：尽管取得了重大进展，但尿液蛋白质组学的诊断尚未完全应用于任何临床环境。这可能归因于肾脏疾病的复杂性，以及研究能力的限制以及将质谱技术纳入日常分析的可行性。尽管如此，我们相信，尿蛋白质组学的进步很快将为肾脏疾病提供超越现有临床参数的卓越见解。