Abstract

The goal was to identify the biofilm-forming ability of Cronobacter sakazakii on surfaces of stainless steel (SS) and silicone rubber (SR) in contact with infant formula milk. Two representative bacteriophages (PBES04 and PBES19) were used to control the growth of C. sakazakii as well as its biofilm forming ability on either SS or SR surfaces. Bacterial growth was confirmed at 20 °C when PBES04 and PBES19 were used, whereas C. sakazakii was not normally detected in infant formula milk treated with both bacteriophages for 6 h. In an additional biofilm reduction experiment, the biofilm on SS or SR surfaces were reduced by 3.07 and 1.92 log CFU cm⁻², respectively after PBES04 treatment, and 3.06 and 2.14 log CFU cm⁻², respectively, after PBES19 treatment. These results demonstrate that bacteriophages can be effective in inactivating C. sakazakii in biofilms which could potentially increase food safety in commercial facilities.

摘要

目的是确定坂崎克罗诺杆菌在与婴儿配方奶接触的不锈钢 (SS) 和硅橡胶 (SR) 表面上的生物膜形成能力。两种代表性的噬菌体（PBES04 和 PBES19）用于控制阪崎肠杆菌的生长及其在 SS 或 SR 表面上的生物膜形成能力。当使用 PBES04 和 PBES19 时，在 20 °C 下确认了细菌生长，而在用两种噬菌体处理 6 小时的婴儿配方奶粉中通常检测不到坂崎梭菌。在额外的生物膜减少实验中，在 PBES04 处理后，SS 或 SR 表面上的生物膜分别减少了 3.07 和 1.92 log CFU cm ^-2，以及 3.06 和 2.14 log CFU cm ^-2分别在 PBES19 处理后。这些结果表明，噬菌体可以有效灭活生物膜中的阪崎肠杆菌，这可能会增加商业设施中的食品安全。