Eggs of Acartia tonsa and Parvocalanus crassirostris were contaminated with ciliates (Euplotes sp.) and exposed to five concentrations of sodium hypochlorite (25, 55, 105, 205, and 405 mg/L) for durations of 15 s, 5 min, 15 min, or 30 min to assess the efficacy of household bleach for removing the ciliates. Eggs from each exposure treatment were incubated for 40 h (A. tonsa) or 12 h (P. crassirostris) to assess hatch. The presence of living ciliates was evaluated after incubation, and the egg hatch rate (%) for the copepods was estimated for each treatment and in controls. Exposure to 105 mg/L total chlorine for 15 s killed all of the ciliates and reduced the copepod hatch rate by less than 26% with respect to controls in both species. Exposure to 55 mg/L for 15 min or 25 mg/L for 30 min also killed the ciliates and achieved a mean copepod hatch rate of 38.5% and 58.1% of the control-group hatch rate, respectively, in A. tonsa. Treatments for 5 min or longer reduced the hatch rate for P. crassirostris eggs by at least 76% at all of the concentrations that we tested.

中文翻译：

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用次氯酸钠去除两种类角足类动物原种中的自由生活纤毛虫

Acartiatonsa和Parvocalanus crassirostris 的卵被纤毛虫 ( Euplotes sp.)污染，并暴露于五种浓度的次氯酸钠（25、55、105、205和405 毫克/升），持续时间为 15 秒、5 分钟、15 分钟或 30 分钟，以评估家用漂白剂去除纤毛虫的功效。来自每个暴露处理的鸡蛋孵化 40 小时（A.tonsa）或 12 小时（P. crassirostris) 来评估孵化。孵化后评估活纤毛虫的存在，并估计每个处理和对照中桡足类的卵孵化率 (%)。与对照相比，暴露于 105 mg/L 总氯 15 秒可杀死所有纤毛虫并将桡足类孵化率降低不到 26%。在 A.tonsa 中，暴露于 55 mg/L 15 分钟或 25 mg/L 30 分钟也会杀死纤毛虫，并分别达到对照组的 38.5% 和 58.1% 的平均孵化率。在我们测试的所有浓度下，5 分钟或更长时间的处理使P. crassirostris卵的孵化率降低了至少 76%。