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Removal of Free-Living Ciliates from Stock Cultures of Two Calanoid Copepods with Sodium Hypochlorite
North American Journal of Aquaculture ( IF 1.4 ) Pub Date : 2021-06-29 , DOI: 10.1002/naaq.10209
Adam L. Daw 1 , Brie L. Sarkisian 1 , Reginald B. Blaylock 1 , Eric A. L. Saillant 1
Affiliation  

Eggs of Acartia tonsa and Parvocalanus crassirostris were contaminated with ciliates (Euplotes sp.) and exposed to five concentrations of sodium hypochlorite (25, 55, 105, 205, and 405 mg/L) for durations of 15 s, 5 min, 15 min, or 30 min to assess the efficacy of household bleach for removing the ciliates. Eggs from each exposure treatment were incubated for 40 h (A. tonsa) or 12 h (P. crassirostris) to assess hatch. The presence of living ciliates was evaluated after incubation, and the egg hatch rate (%) for the copepods was estimated for each treatment and in controls. Exposure to 105 mg/L total chlorine for 15 s killed all of the ciliates and reduced the copepod hatch rate by less than 26% with respect to controls in both species. Exposure to 55 mg/L for 15 min or 25 mg/L for 30 min also killed the ciliates and achieved a mean copepod hatch rate of 38.5% and 58.1% of the control-group hatch rate, respectively, in A. tonsa. Treatments for 5 min or longer reduced the hatch rate for P. crassirostris eggs by at least 76% at all of the concentrations that we tested.

中文翻译:

用次氯酸钠去除两种类角足类动物原种中的自由生活纤毛虫

AcartiatonsaParvocalanus crassirostris 的卵被纤毛虫 ( Euplotes sp.)污染,并暴露于五种浓度的次氯酸钠(25、55、105、205和405 毫克/升),持续时间为 15 秒、5 分钟、15 分钟或 30 分钟,以评估家用漂白剂去除纤毛虫的功效。来自每个暴露处理的鸡蛋孵化 40 小时(A.tonsa)或 12 小时(P. crassirostris) 来评估孵化。孵化后评估活纤毛虫的存在,并估计每个处理和对照中桡足类的卵孵化率 (%)。与对照相比,暴露于 105 mg/L 总氯 15 秒可杀死所有纤毛虫并将桡足类孵化率降低不到 26%。在 A.tonsa 中,暴露于 55 mg/L 15 分钟或 25 mg/L 30 分钟也会杀死纤毛虫,并分别达到对照组的 38.5% 和 58.1% 的平均孵化率。在我们测试的所有浓度下,5 分钟或更长时间的处理使P. crassirostris卵的孵化率降低了至少 76%。
更新日期:2021-06-29
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