ABSTRACT

In this study, a homologous multi-wavelength fluorescence polarization immunoassays (MWFPIA) for rapid determination of amantadine (AMD) and ribavirin (RVB) simultaneously was established. The MWFPIA could quantify AMD and RVB concentration simultaneously in 1 min. After optimisation, long-wavelength tracers AEDA-AF647 with monoclonal antibody (Mab) 1A12 and RVB-CP-EDF with polyclonal antibody (Pab) G412 were chosen for the development of MWFPIA. The limit of detections (LODs) of AMD and RVB were 1.7 and 1.0 μg/kg in chickens, and 17.6 and 10.4 μg/L in human serum, respectively. Recoveries of AMD and RVB in chickens were 61.2–94.9% and 87.3–90.2%, respectively with coefficient of variations (CVs) lower than 16.7%. For human serum, recoveries were 60.2–84.3% and 63.5–74.2% for AMD and RVB, respectively, and CVs was lower than 15.3%. The results indicated that the MWFPIA could be used for the monitoring of antiviral drugs with short analysis time, great accuracy and high throughput.

摘要

在这项研究中，建立了同时快速测定金刚烷胺 (AMD) 和利巴韦林 (RVB) 的同源多波长荧光偏振免疫分析 (MWFPIA)。MWFPIA 可以在 1 分钟内同时量化 AMD 和 RVB 浓度。优化后，长波长示踪剂 AEDA-AF647 与单克隆抗体 (Mab) 1A12 和 RVB-CP-EDF 与多克隆抗体 (Pab) G412 被选择用于开发 MWFPIA。AMD 和 RVB 的检测限 (LOD) 在鸡中分别为 1.7 和 1.0 μg/kg，在人血清中分别为 17.6 和 10.4 μg/L。AMD 和 RVB 在鸡中的回收率分别为 61.2-94.9% 和 87.3-90.2%，变异系数 (CV) 低于 16.7%。对于人血清，AMD 和 RVB 的回收率分别为 60.2-84.3% 和 63.5-74.2%，CV 低于 15.3%。