Rhizospheric bacteria play an indispensable role in plant growth, development, and stress response in plants. This project aims to isolate and functionally characterize plant-growth-promoting rhizobacteria (PGPR) from date palm (Phoenix dactylifera L.) rhizosphere, to investigate its potential uses as a biofertilizer in date palm. Here, we report an Achromobacter xylosoxidans SQU-1 (SQU-1), as a rhizobacteria strain isolated from date palms grown under salinity. The strain was visualized using electron microscopy, identified using protein profiling and 16 S rRNA gene sequencing, functionally characterized, and its genome was sequenced, assembled, and annotated. The results showed that SQU-1 is a Gram-negative flagellated strain and possesses several plant-growth-promoting traits, including the ability to solubilize micronutrients such as zinc, potassium, phosphorus and to produce ammonia, iron siderophores, indole acetic acid (IAA) phytohormone, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase. SQU-1 was also able to significantly enhance date palm seed germination and Arabidopsis growth under normal and saline conditions. Whole-genome sequencing of SQU-1 revealed a total of 6,441,875 bp reads that were assembled into 15 scaffolds. Genome sequence analysis indicated the presence of 5968 open reading frames (ORFs), including 5848 coding genes, 56 pseudogenes, and 64 rRNA DNA sequences. Sequence and cluster of orthologous groups (COG) analysis of SQU-1 enabled the identification of potential genes involved in plant growth-promoting characteristics such as ACC DEAMINASE (acdS), D-CYSTEINE DESULFHYDRASE, and various siderophore production and transporter genes. In conclusion, SQU-1 can be a good candidate for a sustainable agricultural system that aims at producing date fruits under saline conditions with minimal usage of chemical fertilizers.

根际细菌在植物的生长、发育和胁迫反应中起着不可或缺的作用。该项目旨在从枣椰树 ( Phoenix dactylifera L.) 根际中分离出促进植物生长的根际细菌 (PGPR) 并对其进行功能表征，以研究其作为枣椰树生物肥料的潜在用途。在这里，我们报告了木糖氧化无色杆菌SQU-1 (SQU-1)，作为从在盐度下生长的枣椰树中分离的根际细菌菌株。该菌株使用电子显微镜进行可视化，使用蛋白质分析和 16 S rRNA 基因测序进行鉴定，功能表征，并对其基因组进行测序、组装和注释。结果表明，SQU-1 是一种革兰氏阴性鞭毛菌株，具有多种促进植物生长的特性，包括溶解微量营养素如锌、钾、磷和产生氨、铁铁载体、吲哚乙酸 (IAA) 的能力。 ) 植物激素和 1-氨基环丙烷-1-羧酸 (ACC) 脱氨酶。SQU-1 还能够显着增强椰枣种子发芽和拟南芥在正常和盐水条件下生长。SQU-1 的全基因组测序显示总共有 6,441,875 bp 的读数，这些读数组装到 15 个支架中。基因组序列分析表明存在5968个开放阅读框（ORF），包括5848个编码基因、56个假基因和64个rRNA DNA序列。SQU-1 的序列和直系同源群 (COG) 分析能够鉴定涉及植物生长促进特性的潜在基因，例如ACC 脱氨酶( acdS ) 、 D -半胱氨酸脱硫酶，以及各种铁载体生产和转运基因。总之，SQU-1 可以成为可持续农业系统的良好候选者，该系统旨在在盐碱条件下以最少的化肥使用量生产枣果。