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A simple high-volume culture technique—Good substitute for polymerase chain reaction for the detection of Aspergillus species in bronchoalveolar lavage samples
Mycoses ( IF 4.1 ) Pub Date : 2021-06-28 , DOI: 10.1111/myc.13347
Haritha Subhagan 1 , Jayanthi Savio 1 , Priyadarshini Padaki 1 , Sweta Srivastava 2 , Pavana Thomas 2 , Rajeshwari Veerappan 1 , Priya Ramachandran 3 , John Michael Raj Ashok 4
Affiliation  

Aspergillus species is the most common agent of invasive pulmonary fungal disease. Culture-based diagnosis considered as gold standard is limited by the fungal load in samples. Detection of Aspergillus by polymerase chain reaction (PCR) has been included as a diagnostic criterion by European Organisation for Research and Treatment of Cancer (EORTC). Most routine laboratories lack facilities for molecular diagnosis. Better yield using high-volume culture (HVC) technique has been reported. Studies have not compared HVC and PCR for detection of Aspergillus species in respiratory samples from patients with suspected invasive pulmonary Aspergillosis (IPA) not on antifungal therapy.

中文翻译:

一种简单的大容量培养技术——可替代聚合酶链反应检测支气管肺泡灌洗液样品中的曲霉属

曲霉属是侵袭性肺部真菌病最常见的病原体。被认为是金标准的基于培养的诊断受到样本中真菌负荷的限制。通过聚合酶链式反应 (PCR)检测曲霉菌已被欧洲癌症研究和治疗组织 (EORTC) 列为诊断标准。大多数常规实验室缺乏分子诊断设施。据报道,使用大容量培养 (HVC) 技术可提高产量。研究尚未比较 HVC 和 PCR在未接受抗真菌治疗的疑似侵袭性肺曲霉病 (IPA) 患者的呼吸道样本中检测曲霉种类的情况。
更新日期:2021-06-28
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