In this study, ultrasonically driven biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Swietenia macrophylla seed ethyl acetate fraction (SMEAF) has been reported. X-ray powder diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) analyses confirmed the presence of a pure hexagonal wurtzite structure of ZnO. Field emission scanning electron microscope images revealed the formation of uniquely identifiable uniform rice-shaped biologically synthesized ZnO SMEAF particles. The particle sizes of the biosynthesized NPs ranged from 262 to 311 nm. The underlying mechanisms for the biosynthesis of ZnO SMEAF under ultrasound have been proposed based on FTIR and XRD results. The anticancer activity of the as-prepared ZnO SMEAF was investigated against HCT-116 human colon cancer cell lines via methyl thiazolyl tetrazolium assay. ZnO SMEAF exhibited significant anticancer activity against colon cancer cells with higher potency than ZnO particles prepared using the chemical method and SMEAF alone. Exposure of HCT-116 colon cancer cells to ZnO SMEAF promoted a remarkable reduction in cell viability in all the tested concentrations. This study suggests that green sonochemically induced ZnO NPs using medicinal plant extract could be a potential anticancer agent for biomedical applications.

中文翻译：

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用大叶檗籽提取物超声增强均匀 ZnO 纳米大米的生物合成及其体外抗癌活性

在这项研究中，已经报道了使用大叶白杨种子乙酸乙酯组分 (SMEAF) 超声驱动氧化锌纳米粒子 (ZnO NPs) 的生物合成。X 射线粉末衍射 (XRD) 和傅里叶变换红外光谱 (FTIR) 分析证实存在纯六方纤锌矿结构的 ZnO。场发射扫描电子显微镜图像揭示了独特可识别的均匀米状生物合成 ZnO SMEAF 颗粒的形成。生物合成的纳米颗粒的粒径范围为 262 至 311 nm。基于 FTIR 和 XRD 结果提出了超声下 ZnO SMEAF 生物合成的潜在机制。通过甲基噻唑基四唑鎓试验研究了制备的 ZnO SMEAF 对 HCT-116 人结肠癌细胞系的抗癌活性。ZnO SMEAF 对结肠癌细胞表现出显着的抗癌活性，其效力高于使用化学方法制备的 ZnO 颗粒和单独的 SMEAF。HCT-116 结肠癌细胞暴露于 ZnO SMEAF 促进了所有测试浓度的细胞活力显着降低。这项研究表明，使用药用植物提取物的绿色声化学诱导的 ZnO NPs 可能是生物医学应用的潜在抗癌剂。