The overall eradication of biofilm-mode growing bacteria holds significant key to the answer of a series of infection-related health problems. However, the extracellular matrix of bacteria biofilms disables the traditional antimicrobials and, more unfortunately, hampers the development of the anti-infectious alternatives. Therefore, highly effective antimicrobial agents are an urgent need for biofilm-infection control. Herein, a PEGylated palladium nanozyme (Pd-PEG) with peroxidase (POD)-like activity for highly efficient biofilm infection control is reported. Pd-PEG also shows the intrinsic photothermal effect as well as near-infrared (NIR) light-enhanced POD-like activity in the acidic environment, thereby massively destroying the biofilm matrix and killing the adhering bacteria. Importantly, the antimicrobial mechanism of the synergistic treatment based on Pd-PEG+H₂O₂+NIR combination was disclosed. In vitro and in vivo results illustrated the designed Pd-PEG+H₂O₂ +NIR treatment reagent possessed outstanding antibacterial and biofilms elimination effects with negligible biotoxicity. This work hopefully facilitates the development of metal-based nanozymes in biofilm related infectious diseases.

中文翻译：

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近红外光增强了聚乙二醇化钯纳米酶的类过氧化物酶活性，用于高效清除生物膜。

全面根除生物膜模式生长细菌是解决一系列与感染相关的健康问题的关键。然而，细菌生物膜的细胞外基质使传统的抗菌剂失效，更不幸的是，阻碍了抗感染替代品的发展。因此，迫切需要高效的抗菌剂来控制生物膜感染。本文报道了一种具有过氧化物酶 (POD) 样活性的聚乙二醇化钯纳米酶 (Pd-PEG)，用于高效控制生物膜感染。Pd-PEG 在酸性环境中还显示出固有的光热效应以及近红外 (NIR) 光增强的 POD 样活性，从而大量破坏生物膜基质并杀死粘附的细菌。重要的，体外和体内结果表明，设计的 Pd-PEG+H2O2+NIR 处理试剂具有出色的抗菌和生物膜消除效果，生物毒性可忽略不计。这项工作有望促进金属基纳米酶在生物膜相关传染病中的发展。