Reactivity and Selectivity Principles in Native Protein Bioconjugation,The Chemical Record

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Reactivity and Selectivity Principles in Native Protein Bioconjugation
The Chemical Record ( IF 7.0 ) Pub Date : 2021-06-28 , DOI: 10.1002/tcr.202100108
Ramesh Adakkattil ₁ , Kalyani Thakur ₁ , Vishal Rai ₁

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Are chemical methods capable of precisely engineering the native proteins? Is it possible to develop platforms that can empower the regulation of chemoselectivity, site-selectivity, modularity, protein-specificity, and site-specificity? This account delineates our research journey in the last ten years on the developments revolving around these questions. It will range from the realization of chemoselective and site-selective labeling of reactivity hotspots to modular linchpin directed modification (LDM®) platform and site-specific Gly-tag® technology. Also, we outline a few biotechnology tools, including Maspecter®, that accelerated the detailed analysis of the bioconjugates and rendered a powerful toolbox for homogeneous antibody-drug conjugates (ADCs).

中文翻译：

天然蛋白质生物偶联的反应性和选择性原理

化学方法是否能够精确改造天然蛋白质？是否有可能开发出能够调节化学选择性、位点选择性、模块性、蛋白质特异性和位点特异性的平台？这个帐户描述了我们在过去十年中围绕这些问题的发展的研究历程。它的范围从实现反应性热点的化学选择性和位点选择性标记到模块化关键定向修饰 (LDM®) 平台和位点特异性 Gly-tag® 技术。此外，我们还概述了一些生物技术工具，包括 Maspecter®，它们加速了生物偶联物的详细分析，并为均质抗体药物偶联物 (ADC) 提供了强大的工具箱。

更新日期：2021-08-16

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