This paper describes a simplified affinity precipitation process for the purification of mAbs from complex mixtures using elastin-like polypeptide fused to a single Z domain of protein A (ELP-Z). This approach eliminates several steps in the original process by directly extracting the mAb from the affinity precipitate, without the need for resolubilization. The efficacy of this elution without resolubilization (EWR) approach for obtaining pure mAb is demonstrated and the effects of mixing are examined. This simplification of the affinity precipitation process may facilitate the implementation of ELP-Z based mAb bioprocessing, particularly in a continuous scenario.

本文介绍了一种简化的亲和沉淀过程，用于使用与蛋白 A (ELP-Z) 的单个 Z 结构域融合的弹性蛋白样多肽从复杂混合物中纯化 mAb。这种方法直接从亲和沉淀中提取 mAb，无需再溶解，从而消除了原始过程中的几个步骤。证明了这种无需再溶解 (EWR) 的洗脱方法对获得纯 mAb 的功效，并检查了混合的影响。这种亲和沉淀过程的简化可能有助于实施基于 ELP-Z 的 mAb 生物加工，特别是在连续情况下。