ABSTRACT

Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta (cassava), Zea mays (maize), Oryza sativa (rice), Triticum aestivum (wheat), Sorghum bicolor (sorghum), Brassica rapa (rape seed), Solanum tuberosum (potato), Solanum lycopersicum (tomato), Elaes guineensis (oil palm), Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs.

摘要

通过有效的基因转移可以促进作物改良，从而导致 pRAP 质粒的发展。来自 24 篇先前发表的文章检查 29,756 个根的比较毛根转化结果显示了 70% 的转化效率。在 RNAi 根中，平均基因过表达为 11.24 倍和 -3.84 倍。新研究表明，Glycine max BRI1 相关受体激酶 1 (BAK1) 过度表达导致异虫甘氨酸寄生减少 67%，而BAK1-1 RNAi 导致寄生增加 4.8 倍。结果显示G. max-H 中病原体相关的分子模式触发免疫 (PTI) 功能。甘氨酸防御期间的病理系统。因此，pRAP 载体可用于研究其他农业植物的基础生物学和防御，包括Manihot esculenta（木薯）、Zea mays（玉米）、Oryza sativa（水稻）、Triticum aestivum（小麦）、Sorghum bicolor（sorghum）、Brassica rapa（油菜籽）、Solanum tuberosum（马铃薯）、Solanum lycopersicum（番茄）、Elaes guineensis（油棕）、Saccharum officinalis（甘蔗）和Beta vulgaris（甜菜），因为它们各自具有 BAK1 同源物。