This study assessed biofilm production ability (BPA) and other microbiological features of Streptococcus canis strains. Companion animal-origin 40 strains from each year (2015/2017) were randomly selected with the host information, and three blood-origin strains from 2 humans/1 dog were included. We measured BPA using crystal violet staining, along with S. canis M-like protein (SCM) allele typing, sequence type (ST) determination, antimicrobial resistance (AMR) phenotyping/genotyping, and virulence-associated gene profiling (gbp-ap1-fp1-brp). BPA measurements revealed that 35 strains with BPA and 48 strains without BPA. There was association of the producer with isolation year (2017). We found association between the non-producer and SCM allele 1/ST9: there was association of the producer with SCM allele 10/ST21. We observed correlation between the producer and presence of AMR genotypes. There was association between the producer and ap1 detection and between non-producer and gbp detection. Our observations suggest the correlation between the producer and other microbiological features (isolation year/SCM allele type 10/ST21/presence of AMR genotypes/ap1 detection).

本研究评估了犬链球菌 菌株的生物膜生产能力 (BPA) 和其他微生物学特征。根据宿主信息随机选择每年（2015/2017）的伴侣动物来源的40株，其中包括来自2人/1只狗的3株血源菌株。我们使用结晶紫染色以及犬链球菌M 样蛋白 (SCM) 等位基因分型、序列类型 (ST) 测定、抗菌素耐药性 (AMR) 表型/基因分型和毒力相关基因分析 ( gbp-ap1- fp1-brp）。BPA 测量显示，35 株含有 BPA，48 株不含 BPA。生产者与隔离年（2017 年）有关联。我们发现非生产者与 SCM 等位基因 1/ST9 之间存在关联：生产者与 SCM 等位基因 10/ST21 存在关联。我们观察到生产者与 AMR 基因型存在之间的相关性。生产者与ap1检测之间以及非生产者与gbp检测之间存在关联。我们的观察表明生产者与其他微生物特征（分离年份/SCM 等位基因 10 型/ST21/存在 AMR 基因型/ ap1检测）之间的相关性。