This study was conducted under the Italian Ministerial Decree D.M. 173/2016 which regulates the assessment of the Sediment Class Quality in Italy using ecotoxicological bioassay and chemical analysis (Weight-Of-Evidence model). The aim of this work was to evaluate the real classification obtained by the theoretically equivalent responses of nine different combinations of batteries based on six different species: Aliivibrio fischeri (inhibition of bioluminescence), Phaeodactylum tricornutum, Skeletonema costatum, Dunaliella tertiolecta (inhibition of algal growth), Paracentrotus lividus and Crassostrea gigas (embryotoxicity).

Bioassays, in many cases, showed a non-bioavailability effect of the pollutants; these one highly revealed by the chemical analyses. Algal species showed responses very similar from each other. Otherwise, species used for embryotoxicity produced wide responses, consequently modifying the quality class of sediments and the handling management (i.e. landfill confinement or beach nourishment) allowed by the Law.

本研究是根据意大利部长令 DM 173/2016 进行的，该法令规定了使用生态毒理学生物测定和化学分析（证据权重模型）对意大利沉积物等级质量的评估。这项工作的目的是评估通过基于六种不同物种的九种不同电池组合的理论等效响应获得的真实分类：Aliivibrio fischeri（抑制生物发光）、Phaeoodactylum tricornutum、Skeletonema costatum、Dunaliella tertiolecta（抑制藻类生长） )、Paracentrotus lividus 和 Crassostrea gigas（胚胎毒性）。

在许多情况下，生物测定显示污染物的非生物利用度效应；化学分析高度揭示了这一点。藻类物种表现出彼此非常相似的反应。否则，用于胚胎毒性的物种会产生广泛的反应，因此改变了法律允许的沉积物质量等级和处理管理（即垃圾填埋场限制或海滩营养）。