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Virulence associated gene profiling and antimicrobial resistance pattern of Streptococcus suis isolated from clinically healthy pigs from North East India
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-06-28 , DOI: 10.1111/lam.13531
S Rajkhowa 1 , J B Rajesh 2
Affiliation  

This study revealed the prevalence of Streptococcus suis in 20·39% clinically healthy pigs from North East India. All these isolates were screened for the presence of virulence- associated genes such as suilysin (sly), muramidase released protein (mrp), extracellular protein factor (epf) and arginine deiminase (arcA). Of these 62 isolates, 29 isolates carried mrp gene, 17 isolates carried sly gene, 57 isolates carried arcA gene, whereas all isolates were negative for epf gene. The most prevalent genotype was mrp sly epf arcA+ (45·16%) followed by genotypes mrp+ sly epf arcA+ (27·41%), mrp+ sly+ epf arcA+ (19·35%) and mrp sly+ epf arcA (8·06%). High frequency of resistance was observed for antimicrobials such as tetracycline (93·54%), clindamycin (91·93%), co-trimoxazole (88·70%) and erythromycin (85·48%). Antimicrobial resistance patterns of the S. suis isolates revealed 16 resistance groups (R1 to R16), where 93·54% isolates showed multi-drug resistance (≥3 antimicrobial agents). It has also been observed that 57 (91·93%) isolates were resistant to at least four antimicrobials. The most predominant resistance pattern observed was CD-COT-E-TE, which accounted for 38·70% of the isolates. The occurrence of relatively high levels of resistance of S. suis to some antimicrobials (e.g., macrolides, tetracyclines, and sulphonamides) as observed in this study may represent a human health concern. In addition, a relatively higher percentage of S. suis isolated from clinically healthy pigs indicates a carrier status with risk of dissemination to other pigs in the herd as well as to humans.

中文翻译:

从印度东北部临床健康猪中分离的猪链球菌的毒力相关基因分析和抗菌素耐药模式

该研究揭示了来自印度东北部的 20·39% 临床健康猪中的猪链球菌患病率。筛选所有这些分离株中是否存在毒力相关基因,例如水溶素 ( sly )、胞壁酸酶释放蛋白 ( mrp )、细胞外蛋白因子 ( epf ) 和精氨酸脱亚胺酶 ( arcA )。在这62株分离物中,29株携带mrp基因,17株携带sly基因,57株携带arcA基因,而所有分离株的epf基因均为阴性。最普遍的基因型是mrp sly epf arcA + (45·16%) 其次是基因型mrp + sly epf arcA + (27·41%)、mrp + sly + epf arcA + (19·35%) 和mrp sly + epf arcA ( 8·06%)。观察到对四环素 (93·54%)、克林霉素 (91·93%)、复方新诺明 (88·70%) 和红霉素 (85·48%) 等抗微生物药物的耐药频率很高。猪链球菌的抗微生物药物耐药性模式分离株显示 16 个耐药组(R1 至 R16),其中 93·54% 的分离株表现出多重耐药性(≥3 种抗菌剂)。还观察到 57 (91·93%) 个分离株对至少四种抗菌素具有抗性。观察到的最主要的耐药模式是 CD-COT-E-TE,占分离株的 38·70%。本研究中观察到的猪链球菌对某些抗微生物剂(例如大环内酯类、四环素类和磺胺类)产生相对较高水平的耐药性可能代表了人类健康问题。此外,从临床健康猪中分离出的猪链球菌的百分比相对较高,表明其处于携带者状态,存在传播给猪群中其他猪以及人类的风险。
更新日期:2021-06-28
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