Abstract

Increased discharge of dyes into the environment and consequent contamination of water bodies have been issues of public health concern due to the toxicity of dyes. In this study, the ability of Stenotrophomonas sp. CFB-09 to decolorize lignin-mimicking dyes [Congo red (CR), toluidine blue (TB), methyl blue (MB), remazol brilliant blue R (RBBR) and indigo carmine (IC)] and produce ligninolytic enzymes were investigated. Dye decolorization activity was carried out qualitatively on plates and quantitatively by UV–Vis spectrophotometric analysis of broths. Influence of process parameters (initial pH, incubation temperature and media composition) on dye decolorization were also determined. Identification of biodegradation products was done by GC-MS. Results revealed that Stenotrophomonas sp. CFB-09 decolorized dyes by concomitantly producing ligninolytic enzymes and achieved ≥90% decolorization of 25 mg/L CR, MB, RBBR, and IC and 75.0–86.5% decolorization of 100 mg/L of IC, CR and MB at 120 h. Lignin peroxidase exhibited the highest activity among ligninolytic enzymes studied on all dyes. Optimum decolorization for all dyes was achieved at pH 8.0 and 35 °C in the presence of oil palm empty fruit bunch (OPEFB). Findings show that the ligninolytic bacterium, Stenotrophomonas sp. CFB-09 has potentials for application in bioremediation of dye containing effluents.

摘要

由于染料的毒性，染料排放到环境中的增加以及随之而来的水体污染已成为公众健康关注的问题。在这项研究中，窄食单胞菌的能力。研究了 CFB-09 脱色木质素模拟染料 [刚果红 (CR)、甲苯胺蓝 (TB)、甲基蓝 (MB)、雷马唑亮蓝 R (RBBR) 和靛蓝胭脂红 (IC)] 并产生木质素分解酶。染料脱色活性在板上定性地进行，并通过肉汤的紫外-可见分光光度法定量地进行。还确定了工艺参数（初始 pH 值、孵育温度和培养基组成）对染料脱色的影响。生物降解产物的鉴定通过 GC-MS 完成。结果表明，窄食单胞菌sp。CFB-09通过同时产生木质素分解酶对染料进行脱色，25 mg/L CR、MB、RBBR和IC的脱色率≥90%，120 h时100 mg/L的IC、CR和MB脱色率达到75.0-86.5%。在所有染料研究的木质素分解酶中，木质素过氧化物酶的活性最高。在油棕空果串 (OPEFB) 存在下，所有染料的最佳脱色均在 pH 8.0 和 35 °C 下实现。研究结果表明，木质素分解细菌 Stenotrophomonas sp。CFB-09 在含染料废水的生物修复中具有应用潜力。