Plant available phosphorus (P) is limited in the vast rice-fallow areas where the soil is mainly acidic. Screening of fifty lentil genotypes in soil cylinder filled with solid-phase P-buffer identified genotypes with high P-acquisition ability even though desorbed P was 100 μmol/g only. Genotypes with high P-acquisition ability were identified based on their almost equal P-acquisition ability both in P-deficient and sufficient conditions. Root dry weight and root surface area showed a significant positive correlation with shoot phosphate content in P-deficiency condition, but not in P-sufficiency condition. Identified top three tolerant genotypes, namely IC560183, WBL77, and BM3 showed a 30% reduced seed yield in P-deficient field as compared to 60% or more reduction by intolerant genotypes. In P-Deficiency condition, 4–6 times high intercellular acid phosphatase activity in roots of the tolerant genotypes may be one of the factors for high P-acquisition. Higher external secretion of acid phosphatase in tolerant genotypes was also confirmed by blue colour through 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) staining. P-deficiency tolerant lentil genotypes will be useful in expansion and enhancing the productivity of lentil in vast rice-fallow areas, mostly where plant-available P is low.

在土壤主要呈酸性的广阔稻田区，植物有效磷 (P) 是有限的。在充满固相 P 缓冲液的土壤圆柱体中筛选 50 个小扁豆基因型，鉴定出具有高 P 获取能力的基因型，即使解吸的 P 仅为 100 μmol/g。具有高磷获取能力的基因型是根据它们在缺磷和充足条件下几乎相等的磷获取能力来确定的。在缺磷条件下，根干重和根表面积与地上部磷含量呈显着正相关，而在缺磷条件下则不显着。确定的前三个耐受基因型，即 IC560183、WBL77 和 BM3，与不耐受基因型的减少 60% 或更多相比，在缺磷田中的种子产量减少了 30%。在缺磷条件下，耐受基因型根中 4-6 倍的高细胞间酸性磷酸酶活性可能是高磷获取的因素之一。通过 5-溴-4-氯-3-吲哚基-磷酸 (BCIP) 染色，蓝色也证实了耐受基因型酸性磷酸酶的较高外部分泌。耐缺磷扁豆基因型将有助于扩大和提高广大稻田休耕地区的扁豆产量，主要是在植物可用磷含量低的地方。