Soil salinity has been one of the biggest hurdles in achieving better crop yield and quality. Plant growth-promoting rhizobacteria (PGPR) are the symbiotic heterogeneous bacteria that play an important role in the recycling of plant nutrients through phytostimulation and phytoremediation. In this study, bacterial isolates were isolated from salt-polluted soil of Jhajjar and Panipat districts of Haryana, India. The potential salt-tolerant bacteria were screened for their PGPR activities such as phosphate solubilization, hydrogen cyanide (HCN), indole acetic acid (IAA) and ammonia production. The molecular characterization of potent isolates with salt tolerance and PGPR activity was done by 16S rDNA sequencing. Eighteen soil samples from saline soils of Haryana state were screened for salt-tolerant bacteria. The bacterial isolates were analyzed for salt tolerance ranging from 2 to 10%. Thirteen isolates were found salt tolerant at varied salt concentrations. Isolates HB6P2 and HB6J2 showed maximum tolerance to salts at 10% followed by HB4A1, HB4N3 and HB8P1. All the salt-tolerant bacterial isolates showed HCN production with maximum production by HB6J2. Phosphate solubilization was demonstrated by three isolates viz., HB4N3, HB6P2 and HB6J2. IAA production was maximum in HB4A1 (15.89) and HB6P2 (14.01) and least in HB4N3 (8.91). Ammonia production was maximum in HB6P2 (12.3) and least in HB8P1 (6.2). Three isolates HB6J2, HB8P1 and HB4N3 with significant salt tolerance, and PGPR ability were identified through sequencing of amplified 16SrRNA gene and were found to be Bacillus paramycoides, Bacillus amyloliquefaciens and Bacillus pumilus, respectively. The salt-tolerant plant growth-promoting rhizobacteria (PGPR) isolated from saline soil can be used to overcome the detrimental effects of salt stress on plants, with beneficial effects of physiological functions of plants such as growth and yield, and overcome disease resistance. Therefore, application of microbial inoculants to alleviate stresses and enhance yield in plants could be a low cost and environmental friendly option for the management of saline soil for better crop productivity.

中文翻译：

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印度哈里亚纳邦盐碱农田具有促进植物生长活性的耐盐细菌的分离与表征

土壤盐分一直是实现更好作物产量和质量的最大障碍之一。植物生长促进根际细菌 (PGPR) 是共生异质细菌，通过植物刺激和植物修复在植物养分循环中发挥重要作用。在这项研究中，从印度哈里亚纳邦 Jhajjar 和 Panipat 地区的盐污染土壤中分离出细菌分离株。筛选了潜在的耐盐细菌的 PGPR 活性，如磷酸盐增溶、氰化氢 (HCN)、吲哚乙酸 (IAA) 和产氨。通过 16S rDNA 测序对具有耐盐性和 PGPR 活性的有效分离株进行分子表征。对来自哈里亚纳邦盐渍土壤的 18 个土壤样品进行了耐盐细菌筛选。分析细菌分离株的耐盐性范围为 2% 至 10%。发现 13 个分离株在不同的盐浓度下具有耐盐性。分离株 HB6P2 和 HB6J2 在 10% 时显示出最大的盐耐受性，其次是 HB4A1、HB4N3 和 HB8P1。所有耐盐细菌分离株均显示出 HCN 产量，HB6J2 产量最高。三个分离株即HB4N3、HB6P2和HB6J2证明了磷酸盐溶解。IAA 产量在 HB4A1 (15.89) 和 HB6P2 (14.01) 中最高，在 HB4N3 (8.91) 中最低。氨生成量在 HB6P2 (12.3) 中最高，在 HB8P1 (6.2) 中最低。通过对扩增的16SrRNA基因进行测序，鉴定出3株具有显着耐盐性和PGPR能力的HB6J2、HB8P1和HB4N3菌株，分别为副真菌芽孢杆菌、解淀粉芽孢杆菌和短小芽孢杆菌。从盐渍土中分离出的耐盐植物促生根际细菌(PGPR)可用于克服盐胁迫对植物的不利影响，有益于植物的生长和产量等生理功能，克服抗病性。因此，应用微生物接种剂来减轻植物的胁迫和提高产量可能是一种低成本和环境友好的选择，用于管理盐渍土壤以提高作物生产力。