During infection and inflammation, a reduced oxygen level clearly affects cellular functions. Oxygen levels during CNS infections are unknown. Here we established and evaluated an in vivo measurement system to characterize the oxygen level in parallel with bacterial numbers (CFU/mL), the cell number and pH level inside the CSF of healthy compared to Streptococcus suis-infected pigs. The animals were anesthetized over a seven-hour period with isoflurane in air/oxygen at physiologic arterial partial pressure of oxygen. Oxygen levels in CSF of anesthetized pigs were compared to euthanized pigs. The detected partial pressure of oxygen in the CSF remained constant in a range of 47–63 mmHg, independent of the infection status (bacterial or cell number). In contrast, the pH value showed a slight drop during infection, which correlated with cell and bacterial number in CSF. We present physiologic oxygen and pH values in CSF during the onset of bacterial meningitis.

中文翻译：

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猪脑脊液体内氧测量以确定中枢神经系统感染期间的生理和病理生理氧值


在感染和炎症期间，氧含量降低明显影响细胞功能。中枢神经系统感染期间的氧气水平尚不清楚。在这里，我们建立并评估了一个体内测量系统，以与健康猪链球菌感染猪相比，与细菌数量 (CFU/mL)、脑脊液内细胞数量和 pH 水平平行表征氧气水平。在生理动脉氧分压下，用空气/氧气中的异氟烷将动物麻醉七小时。将麻醉猪的脑脊液中的氧气水平与安乐死的猪进行比较。检测到的脑脊液中氧分压保持恒定在 47-63 mmHg 范围内，与感染状态（细菌或细胞数量）无关。相反，感染过程中 pH 值略有下降，这与脑脊液中的细胞和细菌数量相关。我们提供了细菌性脑膜炎发作期间脑脊液中的生理氧和 pH 值。