In this study, the diversity of root-nodulating bacteria associated with Pisum sativum L. cultivated in the Eastern and Western Black Sea regions (11 provinces) and three provinces in the Marmara region were investigated. rDNA-ITS RFLP analysis revealed nine groups within the 40 isolates collected in the study. Based on the phylogenetic analysis of the recA, atpD and glnII genes, isolates within the RFLP groups were assigned to the following Rhizobium species; Group-I: R. laguerreae; Groups -II and -IV: R. ruizarguesonis; Groups -III, -V, -VII, -VIII: genospecies-B (gsB) of the R. leguminosarum complex (Rlc); Group-VI: Rlc gsE; and Group-IX: Rlc gsA. From the nodC-RFLP analysis and phylogenetic analysis of the nodC gene, all rhizobial isolates obtained in this study were assigned to the symbiovar. viciae variety of the related Rhizobium species. In this study, Rlc gsB (n: 19) was the species that most commonly caused the nodulation of P. sativum in the northern Anatolia, followed by R. ruizarguesonis (n: 8). Rhizobium laguerreae (n: 5) was a widespread but not common species, and Rlc gsA (n: 3) and Rlc gsE (n: 5) had a distribution limited to the Eastern and Western Black Sea regions, respectively. Overall, results obtained from species diversity analysis, nodC phylogenetic analysis and nodX analysis suggest a common phylogeographic history for pea-nodulating rhizobia in northern Anatolia and western Europe. Separately, this study presents the first reports of R. laguerreae and R. ruizarguesonis from P. sativum root nodules in Turkey and the first report of R. ruizarguesonis outside of Europe.

在这项研究中，调查了黑海东部和西部地区（11 个省）和马尔马拉地区 3 个省种植的与Pisum sativum L.相关的根瘤菌的多样性。rDNA-ITS RFLP 分析揭示了研究中收集的 40 个分离株中的 9 个组。基于recA、atpD和glnII基因的系统发育分析，RFLP 组内的分离株被分配到以下根瘤菌物种；第 I 组：R. laguereae；-II 和 -IV 组：R. ruizarguesonis；基-III，-V，-VII，-VIII：基因种-B的的（GSB）豆科根瘤菌络合物（了Rlc); 第六组：Rlc gsE；和组-IX ：Rlc gsA。从nodC基因的nodC- RFLP 分析和系统发育分析来看，本研究中获得的所有根瘤菌分离株都属于共生变种。相关根瘤菌属的viciae变种。在这项研究中，Rlc gsB ( n : 19) 是最常导致安纳托利亚北部P. sativum结瘤的物种，其次是R. ruizarguesonis ( n : 8)。Rhizobium laguereae ( n : 5) 是一种分布广泛但不常见的物种，RlcgsA ( n : 3) 和Rlc gsE ( n : 5) 的分布分别限于黑海东部和西部地区。总体而言，从物种多样性分析、nodC系统发育分析和nodX分析获得的结果表明，安纳托利亚北部和西欧豌豆根瘤菌具有共同的系统地理历史。另外，这项研究提出的第一次报告R. laguerreae和R. ruizarguesonis从P.豌豆土耳其根瘤和第一报告R. ruizarguesonis欧洲以外的地区。