Background

Obesity has been linked to DNA damage. The modifiable risk factors may modulate the impact of obesity on DNA damage.

Objective

This study aimed to assess DNA damage and its association with dietary nutrient, serum 25-hydroxyvitamin D (25(OH)D) and concentration of hair heavy metals of obese and non-obese women.

Method

A case–control study was conducted involving 134 women aged between 20 and 50 years. Serum 25(OH)D, fasting glucose, and lipid profile were assessed. Indicators of DNA damage such as percentage of tail DNA, tail moment, tail olive moment, tail intensity and tail length were measured using an alkaline-comet assay. Concentrations of hair heavy metals were quantified using inductively coupled plasma–mass spectrometry (ICP–MS). Participants' daily energy, macro, and micronutrient intake were collected using the Food Frequency Questionnaire.

Results

Mean values of serum 25(OH)D was 31.8 ± 0.9 nmol/L. 96.3% of participants were vitamin D deficiency (< 50 nmol/L). The mean BMI was 26.3 ± 0.5 kg/m². Half of the participants (50.7%) have a high frequency of DNA strand breaks. Mean concentration of hair heavy metals (mg/kg) were 0.1 ± 0.03 (arsenic), 0.2 ± 0.1 (cadmium), 1.0 ± 0.4 (mercury), 2.8 ± 0.8 (lead),and 6.2 ± 0.4 (chromium). There was no significant difference for the mean of serum 25(OH)D, indicators of DNA damage, concentrations of hair heavy metals and dietary nutrients between obese and non-obese groups (p > 0.05). Obese women with serum 25(OH)D level of ≥ 31 nmol/L had a significantly lower tail moment (p = 0.029) and tail olive moment (p = 0.031); thus, indicating less DNA damage. Additionally, obese women with hair chromium concentration of ≥ 5.88 mg/kg had a significantly higher tail moment (p = 0.047), indicating more DNA damage.

Conclusion

DNA damage among obese women correlated with serum 25(OH)D and hair chromium.

中文翻译：

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DNA损伤与肥胖女性维生素D和头发重金属的关系

背景

肥胖与 DNA 损伤有关。可改变的风险因素可能会调节肥胖对 DNA 损伤的影响。

客观的

本研究旨在评估 DNA 损伤及其与饮食营养、血清 25-羟基维生素 D (25(OH)D) 和肥胖和非肥胖女性头发重金属浓度的关系。

方法

一项病例对照研究涉及 134 名年龄在 20 至 50 岁之间的女性。评估了血清 25(OH)D、空腹血糖和血脂状况。使用碱性彗星试验测量 DNA 损伤的指标，例如尾 DNA 的百分比、尾矩、尾橄榄矩、尾强度和尾长。使用电感耦合等离子体质谱法 (ICP-MS) 量化头发重金属的浓度。使用食物频率问卷收集参与者的每日能量、宏量和微量营养素摄入量。

结果

血清 25(OH)D 的平均值为 31.8 ± 0.9 nmol/L。96.3% 的参与者缺乏维生素 D (< 50 nmol/L)。平均 BMI 为 26.3 ± 0.5 kg/m ²。一半的参与者 (50.7%) 的 DNA 链断裂频率很高。头发重金属的平均浓度 (mg/kg) 为 0.1 ± 0.03（砷）、0.2 ± 0.1（镉）、1.0 ± 0.4（汞）、2.8 ± 0.8（铅）和 6.2 ± 0.4（铬）。肥胖组与非肥胖组的血清 25(OH)D 均值、DNA 损伤指标、毛发重金属浓度和膳食营养素含量无显着差异（p  > 0.05）。血清 25(OH)D 水平 ≥ 31 nmol/L 的肥胖女性的尾矩 ( p  = 0.029) 和尾橄榄矩 ( p = 0.031); 因此，表明 DNA 损伤较少。此外，头发铬浓度 ≥ 5.88 mg/kg 的肥胖女性具有显着更高的尾矩 ( p  = 0.047)，表明更多的 DNA 损伤。

结论

肥胖女性的 DNA 损伤与血清 25(OH)D 和头发铬相关。