Abstract

Aim: Cisplatin is a widely used and highly effective anti-cancer agent and one of the limiting side effects of cisplatin is ocular toxicity. Achillea millefolium, also known as yarrow, is a plant that has been used for many years to treat various health problems including chemotherapy-related toxicities.

Methods: The present investigation was designed to evaluate the biochemical, molecular and histopathological effects of Achillea Millefolium on cisplatin-induced oxidative and inflammatory ocular damage in rats. Twenty-four adult male rats were assigned randomly to four groups (n = 6) as (1) control, (2) cisplatin (7 mg/kg, intraperitoneally), (3) Cisplatin + Achillea millefolium (200 mg/kg, orally for 14 consecutive days), (4) Cisplatin + Achillea millefolium (400 mg/kg, orally for 14 consecutive days). Levels of total antioxidant capacity and total oxidant status, SOD, MDA, IL-1β, and IL-10 were measured in ocular tissue. The mRNA expressions of TNF-α, nuclear factor kappa B and Caspase-3 were evaluated. Also, ocular sections were evaluated histopathologically.

Results: Achillea Millefolium upregulated ocular antioxidant enzymes and downregulated inflammation. The SOD activity and total antioxidant capacity increased whereas total oxidant status and MDA levels decreased significantly at high dose group. High dose Achillea millefolium treatment reduced the IL-1β concentrations, whereas IL-10 levels increased significantly in that group. Moreover, we observed that Achillea millefolium restored ocular histopathological structure and significantly suppressed apoptosis by reducing the expression of Caspase-3.

Conclusion: Collectively, our results suggest that Achillea millefolium have protective effects against cisplatin-induced ocular toxicity and is a promising adjuvant therapy with the potential to prevent cisplatin related ocular toxicity.

摘要

目的：顺铂是一种广泛使用且高效的抗癌剂，顺铂的限制性副作用之一是眼部毒性。蓍草，也称为蓍草，是一种多年来一直用于治疗各种健康问题的植物，包括与化疗相关的毒性。

方法：本研究旨在评估蓍草对顺铂诱导的大鼠氧化和炎症性眼损伤的生化、分子和组织病理学影响。将 24 只成年雄性大鼠随机分配到四组 ( n = 6 ) 作为 (1) 对照组、(2) 顺铂 (7 mg/kg, 腹膜内), (3) 顺铂 +蓍草(200 mg/kg, 口服)连续14天），（4）顺铂+蓍草（400 mg/kg，连续 14 天口服）。在眼组织中测量总抗氧化能力和总氧化状态、SOD、MDA、IL-1β和IL-10的水平。评估TNF-α、核因子κB和Caspase-3的mRNA表达。此外，对眼部切片进行组织病理学评估。

结果：Achillea Millefolium上调眼部抗氧化酶并下调炎症。高剂量组 SOD 活性和总抗氧化能力增加，而总氧化状态和 MDA 水平显着降低。高剂量蓍草处理降低了 IL-1β 浓度，而 IL-10 水平在该组中显着增加。此外，我们观察到蓍草通过降低 Caspase-3 的表达来恢复眼组织病理学结构并显着抑制细胞凋亡。

结论：总的来说，我们的研究结果表明蓍草对顺铂诱导的眼部毒性具有保护作用，是一种很有前景的辅助疗法，具有预防顺铂相关眼部毒性的潜力。