Unlike mammals, two kisspeptins genes encoding, kiss1 and kiss2 are detected in fishes with highly varied and contradictory difference in their reproductive activities. The present study was undertaken to examine the direct action of kisspeptin-10 and its role in gonadal activities in the gonadally quiescent Asian catfish using native mammalian kisspeptin decapeptide (KP-10) involving in vivo and in vitro approaches. The in vivo KP-10 treatment caused precocious onset of gametogenesis and its rapid progression, as was evident from the appearance of advanced stages of ovarian follicles in ovary, and advanced germ cells (spermatocytes/ spermatids) in the testis of the treated Clarias batrachus in comparison to the control gonads. It also elevated the steroid levels in gonads of the catfish in vivo and in vitro conditions. Simultaneously, it increased the expressions of key steroidogenic enzymes like 3β-HSD, 17β-HSD, and StAR protein, responsible for transfer of cholesterol from outer to inner membrane of the mitochondria of steroidogenic cells. Concurrently, it augmented the activities of 3β-HSD and 17β-HSD in the ovarian explants. The expressions of MAPK component (pERK1/2 and ERK1/2) were also up-regulated by KP-10 in gonadal explants. Thus, the data suggest that kisspeptin-10 stimulates gametogenesis by enhancing gonadal steroid production. The study also describes the putative mechanistic cascade of steroidogenic actions of kisspeptin-10 in the catfish so much so in teleost fish. The study also suggests that, kisspeptin may act locally to regulate gonadal activities in an autocrine/paracine manner, independent of known extra-gonadal factors in the catfish.

与哺乳动物不同的是，在鱼类中检测到两个kisspeptins 基因kiss1和kiss2，它们的繁殖活动具有高度多样性和矛盾的差异。本研究旨在使用本地哺乳动物 Kisspeptin 十肽 (KP-10) 涉及体内和体外方法来检查kisspeptin-10 的直接作用及其在性腺静止的亚洲鲶鱼性腺活动中的作用。的体内KP-10处理导致配子和其快速进展的早熟发作，因为是从卵巢滤泡的晚期卵巢外观明显的，并且在经处理的睾丸先进生殖细胞（精母细胞/精细胞）胡子蟾与对照性腺相比。它还在体内和体外提高了鲶鱼性腺中的类固醇水平状况。同时，它增加了关键类固醇生成酶的表达，如 3β-HSD、17β-HSD 和 StAR 蛋白，这些酶负责胆固醇从类固醇生成细胞线粒体的外膜到内膜的转移。同时，它增强了卵巢外植体中 3β-HSD 和 17β-HSD 的活性。MAPK 组分（pERK1/2 和 ERK1/2）的表达也被 KP-10 在性腺外植体中上调。因此，数据表明kisspeptin-10 通过增强性腺类固醇的产生来刺激配子发生。该研究还描述了 Kisspeptin-10 在鲶鱼中的类固醇生成作用的假定机制级联，在硬骨鱼中是如此。该研究还表明，kisspeptin 可能在局部以自分泌/旁分泌方式调节性腺活动，