Abstract

Vibrational spectroscopic techniques, namely Fourier transform infrared (FTIR) and Raman spectroscopy, are based on the study of molecular vibrations, and they are complementary techniques to each other. This review provides an overview of the vibrational spectroscopic techniques applied in microbiology during the past decade. In addition, future applications of the elaborated spectroscopic techniques will be highlighted. The results of this review show that both FTIR and Raman spectroscopy are promising alternatives to conventional diagnostic approaches because they provide label-free and noninvasive bacterial detection, identification, and antibiotic susceptibility testing in a single step. Cost-effective, accurate, and rapid tests are needed in order to improve diagnostics and patient care, to decrease the use of unnecessary antimicrobial agents, to prevent resistant microbials, and to decrease the overall burden of outbreaks. Prior to that, however, the presented approaches need to be validated in a clinical workflow against the conventional diagnostic approaches.

摘要

振动光谱技术，即傅里叶变换红外光谱（FTIR）和拉曼光谱，是基于对分子振动的研究，它们是相互补充的技术。本综述概述了过去十年在微生物学中应用的振动光谱技术。此外，还将重点介绍详细光谱技术的未来应用。这篇综述的结果表明，FTIR 和拉曼光谱都是传统诊断方法的有前途的替代方法，因为它们一步提供无标记和无创的细菌检测、鉴定和抗生素敏感性测试。为了改进诊断和患者护理，需要具有成本效益、准确和快速的测试，减少不必要的抗菌剂的使用，防止耐药微生物，并降低疾病暴发的总体负担。然而，在此之前，所提出的方法需要在临床工作流程中针对传统诊断方法进行验证。