ABSTRACT

The growing prevalence of vascular diseases worldwide has emphasized the need for novel tissue-engineered options concerning the development of vascularized 3D constructs. This study reports, for the first time, the use of external magnetic fields to stimulate mesenchymal stromal cells (MSCs) to increase the production of vascular endothelial growth factor-A (VEGF-A). Polyvinylalcohol and gelatin-based scaffolds, containing iron oxide nanoparticles, were designed for optimal cell magnetic stimulation. While the application of static magnetic fields over 24 h did not impact on MSCs proliferation, viability and phenotypic identity, it significantly increased the production of VEGF-A and guided MSCs morphology and alignment. The ability to enhance MSCs angiogenic potential was demonstrated by the increase in the number of new vessels formed in the presence of MSCs conditioned media through in vitro and in vivo models. Ultimately, this study uncovers the potential to manipulate cellular processes through short-term magnetic stimulation.

 抽象的

全球血管疾病的日益流行强调了对有关血管化 3D 结构开发的新型组织工程选择的需求。这项研究首次报告，利用外部磁场刺激间充质基质细胞（MSC）以增加血管内皮生长因子-A（VEGF-A）的产生。含有氧化铁纳米颗粒的聚乙烯醇和明胶支架被设计用于最佳的细胞磁刺激。虽然施加超过 24 小时的静磁场不会影响 MSC 的增殖、活力和表型特性，但它显着增加了 VEGF-A 的产生并引导 MSC 的形态和排列。通过体外和体内模型，在 MSC 条件培养基存在下形成的新血管数量增加，证明了增强 MSC 血管生成潜力的能力。最终，这项研究揭示了通过短期磁刺激操纵细胞过程的潜力。