Heart formation in the zebrafish involves a rapid, complex series of morphogenetic events in three-dimensional space that spans cardiac lineage specification through to chamber formation and maturation. This process is tightly orchestrated by a cardiac gene regulatory network (GRN), which ensures the precise spatio-temporal deployment of genes critical for heart formation. Alterations of the timing or spatial localisation of gene expression can have a significant impact in cardiac ontogeny and may lead to heart malformations. Hence, a better understanding of the cellular and molecular basis of congenital heart disease relies on understanding the behaviour of cardiac GRNs with precise spatiotemporal resolution. Here, we review the recent technical advances that have expanded our capacity to interrogate the cardiac GRN in zebrafish. In particular, we focus on studies utilising high-throughput technologies to systematically dissect gene expression patterns, both temporally and spatially during heart development.

中文翻译：

---

斑马鱼时空解析的发育心脏基因调控网络


斑马鱼心脏的形成涉及三维空间中一系列快速、复杂的形态发生事件，涵盖心脏谱系规范直至心室形成和成熟。这一过程由心脏基因调控网络（GRN）紧密协调，确保对心脏形成至关重要的基因的精确时空部署。基因表达的时间或空间定位的改变可能对心脏个体发育产生重大影响，并可能导致心脏畸形。因此，更好地了解先天性心脏病的细胞和分子基础依赖于以精确的时空分辨率了解心脏 GRN 的行为。在这里，我们回顾了最近的技术进步，这些进步扩大了我们询问斑马鱼心脏 GRN 的能力。特别是，我们专注于利用高通量技术系统地剖析心脏发育过程中时间和空间上的基因表达模式的研究。