Deletion of Rap-Phr systems in Bacillus subtilis influences in vitro biofilm formation and plant root colonization,MicrobiologyOpen

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Deletion of Rap-Phr systems in Bacillus subtilis influences in vitro biofilm formation and plant root colonization
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-06-27 , DOI: 10.1002/mbo3.1212
Mathilde Nordgaard ₁ , Rasmus Møller Rosenbek Mortensen ₁ , Nikolaj Kaae Kirk ₁ , Ramses Gallegos-Monterrosa ₂ , Ákos T Kovács ₁

Affiliation

Natural isolates of the soil-dwelling bacterium Bacillus subtilis form robust biofilms under laboratory conditions and colonize plant roots. B. subtilis biofilm gene expression displays phenotypic heterogeneity that is influenced by a family of Rap-Phr regulatory systems. Most Rap-Phr systems in B. subtilis have been studied independently, in different genetic backgrounds and under distinct conditions, hampering true comparison of the Rap-Phr systems’ impact on bacterial cell differentiation. Here, we investigated each of the 12 Rap-Phr systems of B.subtilis NCIB 3610 for their effect on biofilm formation. By studying single ∆rap-phr mutants, we show that despite redundancy between the cell–cell communication systems, deletion of each of the 12 Rap-Phr systems influences matrix gene expression. These Rap-Phr systems therefore enable fine-tuning of the timing and level of matrix production in response to specific conditions. Furthermore, some of the ∆rap-phr mutants demonstrated altered biofilm formation in vitro and colonization of Arabidopsis thaliana roots, but not necessarily similarly in both processes, indicating that the pathways regulating matrix gene expression and other factors important for biofilm formation may be differently regulated under these distinct conditions.

中文翻译：

枯草芽孢杆菌中 Rap-Phr 系统的缺失影响体外生物膜形成和植物根部定植

土壤细菌枯草芽孢杆菌的天然分离物在实验室条件下形成坚固的生物膜并定殖在植物根部。枯草芽孢杆菌生物膜基因表达表现出受 Rap-Phr 调控系统家族影响的表型异质性。枯草芽孢杆菌中的大多数 Rap-Phr 系统都是在不同遗传背景和不同条件下进行独立研究的，这阻碍了 Rap-Phr 系统对细菌细胞分化影响的真正比较。在这里，我们研究了枯草芽孢杆菌NCIB 3610 的 12 个 Rap-Phr 系统中的每一个系统对生物膜形成的影响。通过研究单个 Δ rap - phr突变体，我们发现尽管细胞间通讯系统之间存在冗余，但 12 个 Rap-Phr 系统中每一个的删除都会影响基质基因表达。因此，这些 Rap-Phr 系统能够根据特定条件微调基质产生的时间和水平。此外，一些Δrap - phr突变体表现出体外生物膜形成和拟南芥根部定植的改变，但在这两个过程中不一定相似，表明调节基质基因表达的途径和对生物膜形成重要的其他因素可能受到不同的调节在这些不同的条件下。

更新日期：2021-06-28

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