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Nanoparticles targeting amyloid deposits: a potential contrast agent for diagnosis and treatment
Journal of Nanophotonics ( IF 1.5 ) Pub Date : 2021-06-01 , DOI: 10.1117/1.jnp.15.026010
Michal Tepper 1 , Pinkie J. Eravuchira 1 , Batel Gabay 1 , Orna Sharabani-Yosef 1 , Israel Gannot 1
Affiliation  

Amyloid-based diseases, such as amyloid light-chain amyloidosis, are characterized by misfolding of proteins and their deposition as amyloids in tissues. As prognosis is usually poor, patients suffering from these illnesses can benefit from improved detection, monitoring, and treatment techniques. The use of nanoparticles to diagnose and treat biological targets has been extensively studied, including as a potential marker for Alzheimer’s disease, but not in the context of amyloidosis. Although curcumin is a known amyloid-binding molecule, vanillin attachment to amyloids has not been proposed or tested in the past. Our study focuses on iron oxide and gold nanoparticles, functionalized with curcumin and vanillin, as potential agents for amyloid specific binding. These nanoparticles are designed to have high visibility in computed tomography or magnetic resonance imaging and can therefore facilitate improved imaging and monitoring of amyloids. Amyloid fibers and plaques were prepared from insulin, and the successful binding of the nanoparticles to the amyloids was demonstrated using optical, fluorescence, and transmission electron microscopy. The nanoparticles did not bind to amyloids placed in cell-culture models, suggesting good specificity.

中文翻译:

靶向淀粉样蛋白沉积物的纳米颗粒:一种用于诊断和治疗的潜在造影剂

基于淀粉样蛋白的疾病,例如淀粉样蛋白轻链淀粉样变性,其特征在于蛋白质的错误折叠及其在组织中作为淀粉样蛋白的沉积。由于预后通常很差,患有这些疾病的患者可以从改进的检测、监测和治疗技术中受益。纳米粒子在诊断和治疗生物靶标方面的应用已得到广泛研究,包括作为阿尔茨海默病的潜在标志物,但不是在淀粉样变性的背景下。尽管姜黄素是一种已知的淀粉样蛋白结合分子,但过去没有提出或测试过香草醛与淀粉样蛋白的结合。我们的研究重点是氧化铁和金纳米粒子,用姜黄素和香草醛进行功能化,作为淀粉样蛋白特异性结合的潜在药物。这些纳米粒子被设计为在计算机断层扫描或磁共振成像中具有高可见度,因此可以促进淀粉样蛋白的改进成像和监测。淀粉样蛋白纤维和斑块由胰岛素制备,纳米颗粒与淀粉样蛋白的成功结合通过光学、荧光和透射电子显微镜证明。纳米颗粒不与置于细胞培养模型中的淀粉样蛋白结合,表明具有良好的特异性。
更新日期:2021-06-28
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