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Equilibrium Conformation of Catenated DNA Networks in Slitlike Confinement
ACS Macro Letters ( IF 5.1 ) Pub Date : 2021-06-25 , DOI: 10.1021/acsmacrolett.1c00299
Beatrice W Soh 1 , Patrick S Doyle 1
Affiliation  

A kinetoplast is a planar network of catenated DNA rings with topology that resembles that of chain mail armor. In this work, we use single-molecule experiments to probe the conformation of kinetoplasts confined to slits. We find that the in-plane size of kinetoplasts increases with degree of confinement, akin to the slitlike confinement of linear DNA. The change in kinetoplast size with channel height is consistent with the scaling prediction from a Flory-type approach for a 2D polymer. With an increase in extent of confinement, the kinetoplasts appear to unfold and take on more uniform circular shapes, in contrast to the broad range of conformations observed for kinetoplasts in bulk.

中文翻译:

狭缝限制中链状 DNA 网络的平衡构象

动质体是一个由链状 DNA 环组成的平面网络,其拓扑结构类似于锁子甲。在这项工作中,我们使用单分子实验来探测局限于狭缝的动质体的构象。我们发现运动体的平面内尺寸随着限制程度的增加而增加,类似于线性 DNA 的狭缝限制。动质体尺寸随通道高度的变化与二维聚合物的 Flory 型方法的缩放预测一致。随着限制程度的增加,运动体似乎展开并呈现更均匀的圆形形状,这与在散装运动体中观察到的广泛构象形成对比。
更新日期:2021-07-20
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