Benzo(b)fluoranthene (BbF) is a polycyclic aromatic hydrocarbon (PAH) with five fused benzene rings; it is a highly recalcitrant compound and a priority environmental pollutant due to its detrimental effects on human health and the survival of wild animals. Biodegradation of BbF by microorganisms is an attractive alternative, and few studies have been focused on this issue. In this work, bacteria with the ability to degrade BbF were isolated and selected. The capability of the isolates to tolerate concentrations of 50 and 75 mg l⁻¹ of BbF in liquid medium was evaluated. The selected isolates were identified by the 16S rRNA gene sequencing as belonging to Bacillus, Gordonia, Pseudomonas, Rhodococcus, Ochrobactrum, and Amycolatopsis. All isolates were tolerant and grew at the BbF concentrations tested, some isolates were more competitive than others, and the most prominent was Amycolatopsis sp. Ver12, which removed 47% of BbF, furthermore, with the addition of yeast extract, removed 59% of the compound. In summary, the report shows that Amycolatopsis sp. Ver12 can degrade BbF efficiently and could be considered for bioremediation of BbF-contaminated environments.

中文翻译：

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Amycolaptosis sp. 的评估。Ver12 作为苯并 (b) 荧蒽的潜在降解剂

苯并(b)荧蒽 (BbF) 是具有五个稠合苯环的多环芳烃 (PAH)；由于它对人类健康和野生动物的生存产生不利影响，因此它是一种高度顽固的化合物和优先环境污染物。微生物对 BbF 的生物降解是一种有吸引力的替代方法，很少有研究关注这个问题。在这项工作中，分离和选择了具有降解 BbF 能力的细菌。评估了分离株在液体培养基中耐受浓度为 50 和 75 mg l ^-1的 BbF 的能力。所选择的分离物通过16S rRNA基因测序鉴定为属于芽孢杆菌属，大头，假单胞菌属，红球菌属，Ochrobactrum和Amycolatopsis。所有分离株都耐受并在测试的 BbF 浓度下生长，一些分离株比其他分离株更具竞争力，最突出的是Amycolatopsis sp。Ver12 去除了 47% 的 BbF，此外，通过添加酵母提取物，去除了 59% 的化合物。总之，该报告显示Amycolatopsis sp。Ver12 可以有效降解 BbF，可考虑用于 BbF 污染环境的生物修复。