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Expression Level of Membrane Markers CD5, CD19, and CD20 in B Cells after UV-Irradiation and Incubation in the Presence of Autologous Plasma
Bulletin of Experimental Biology and Medicine ( IF 0.9 ) Pub Date : 2021-06-26 , DOI: 10.1007/s10517-021-05214-3
O V Zemchenkova 1 , O V Basharina 2 , V G Artyukhov 2
Affiliation  

The effect of UV-light (240-390 nm) in doses of 151 and 755 J/m2 on the expression of membrane markers CD5, CD19, CD20 in human peripheral blood B cells was studied by flow cytometry. In 24 h after exposure to UV light, we observed activation of processes accompanied by structural rearrangements of B-cell membranes leading to changes in the expression of receptor molecules: the content of of CD19 and CD20 increased due to activation of the synthesis of these proteins, while the content of CD5 decreased. The percentage of CD5+ cells decreased over 24 h after UV-irradiation of lymphocytes, while addition of autologous plasma to the incubation medium produced a photoprotective effect on CD5+ cells.



中文翻译:

自体血浆存在下紫外线照射和孵育后 B 细胞中膜标记 CD5、CD19 和 CD20 的表达水平

通过流式细胞术研究了151和755 J/m 2剂量的紫外光(240-390 nm)对人外周血B细胞膜标志物CD5、CD19、CD20表达的影响。在暴露于紫外线后 24 小时,我们观察到伴随 B 细胞膜结构重排的过程的激活,导致受体分子表达的变化:CD19 和 CD20 的含量由于这些蛋白质合成的激活而增加, 而 CD5 的含量下降。在对淋巴细胞进行紫外线照射后,CD5 +细胞的百分比在 24 小时内下降,而在培养基中加入自体血浆对 CD5 +细胞产生了光保护作用。

更新日期:2021-06-28
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