This study presents a successful treatment of biological acidic Se(IV)- and Cd(II)-containing wastewater via the SBR with limited carbon source (100 mg/L COD). Scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDS), high solution transmission electron microscopy (HRTEM) and X-ray photoelectron spectrometer (XPS) results verified the formation of elemental Se and CdSe nanoparticles in the sludge. The abundance of genera in the microbial community gradually changed over the treatment phases depending on the Se(IV) and Cd(II) exposure with different influent COD concentrations. The taxa of Proteiniclasticum, Clostridium_sensu_stricto_12, Longilinea and Mycobacterium were dominant. Redundancy analysis (RDA) indicates that COD concentrations had the greatest impact on Zoogloea and Pseudomonas by promoting an increased abundance and decreased abundance, respectively. Overall, the results extended our understanding of the mechanisms and microbial community responding for the Se(IV) and Cd(II) removal under limited carbon availability in acidic wastewater.

本研究展示了通过有限碳源 (100 mg/L COD) 的 SBR 成功处理含硒 (IV) 和镉 (II) 的生物酸性废水。扫描电子显微镜结合能量色散 X 射线光谱 (SEM-EDS)、高溶液透射电子显微镜 (HRTEM) 和 X 射线光电子能谱仪 (XPS) 结果证实了污泥中元素 Se 和 CdSe 纳米颗粒的形成。在不同的进水 COD 浓度下，微生物群落中属的丰度在处理阶段逐渐变化，这取决于 Se(IV) 和 Cd(II) 暴露。Proteiniclasticum , Clostridium_sensu_stricto_12 , Longilinea和分枝杆菌的分类群占主导地位。冗余分析 (RDA) 表明 COD 浓度通过分别促进丰度增加和丰度减少对Zoogloea和假单胞菌的影响最大。总体而言，这些结果扩展了我们对酸性废水中有限碳可用性下去除 Se(IV) 和 Cd(II) 的机制和微生物群落的理解。