The objective of this study was to evaluate the antibacterial effect of sophorolipid in combination with lactic acid against relevant bacteria isolated from the poultry industry. Staphylococcus aureus, Listeria monocytogenes, Salmonella enterica, and Escherichia coli were isolated from chicken meat and antibacterial tests with sophorolipid and lactic acid were performed. Checkerboard, time-kill, and scanning electron microscopy analyses were used to confirm the antibacterial action and the combined effects. Although no inhibitory effects were observed for E. coli and Salmonella, these compounds presented antibacterial activity against L. monocytogenes and S. aureus. Additionally, sophorolipid and lactic acid were not cytotoxic at the concentrations used in the tests. The combination of sophorolipid and lactic acid resulted in an additive interaction, reducing the concentration of the active compounds needed for effectiveness against S. aureus and L. monocytogenes, to 50% and 75%, respectively. These findings lead to the possibility of developing a new, sustainable, and natural antimicrobial solution that is considered noncytotoxic and has wide applicability in the poultry industry to reduce substantial losses in this sector.

本研究的目的是评估槐糖脂与乳酸组合对家禽业分离的相关细菌的抗菌效果。从鸡肉中分离出金黄色葡萄球菌、单增李斯特菌、肠沙门氏菌和大肠杆菌，并用槐糖脂和乳酸进行抗菌试验。使用棋盘、时间杀灭和扫描电子显微镜分析来确认抗菌作用和综合效果。虽然没有观察到对大肠杆菌和沙门氏菌的抑制作用，但这些化合物对单核细胞增生李斯特菌和金黄色葡萄球菌具有抗菌活性。此外，槐糖脂和乳酸在测试中使用的浓度下没有细胞毒性。槐糖脂和乳酸的组合产生了相加的相互作用，将有效对抗金黄色葡萄球菌和单核细胞增生李斯特菌所需的活性化合物浓度分别降低至 50% 和 75%。这些发现使得开发一种新的、可持续的、天然的抗菌解决方案成为可能，该解决方案被认为是无细胞毒性的，并且在家禽业中具有广泛的适用性，以减少该行业的重大损失。