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Quantification of Cannabinoids in Cultivars of Cannabis sp. by Gas Chromatography–Mass Spectrometry
Chromatographia ( IF 1.2 ) Pub Date : 2021-06-27 , DOI: 10.1007/s10337-021-04060-9
Abdul Qudeer Ahmed , David Noshad , Paul C. H. Li

In the chemical characterization of medically valued Cannabis, the present work has used a gas chromatography (GC) method coupled with mass spectrometry (MS) for identification and quantification of cannabinoids. We have modified a GC–MS method for chemical analysis of cannabinoids extracted from dried flowers of different Cannabis varieties. The method allows for quantification of major cannabinoids, i.e. cannabidiol (CBD), cannabichromene (CBC), tetrahydrocannabinol (THC), cannabigerol (CBG), and cannabinol (CBN) simultaneously. We found that the Cannabis cultivar called Cannabis 5-CW had the highest amount of CBD. We used a modified GC–MS method for chemical profiling of cannabinoids extracted from a variety of Cannabis samples, especially the high CBD, CBC, THC, CBG and CBN-producing ones. The method was successfully applied for identification and quantification of cannabinoids in a short time with better separation resolution among the reported methods.



中文翻译:

大麻品种中大麻素的定量。通过气相色谱-质谱法

在具有医学价值的大麻的化学表征中,目前的工作使用气相色谱 (GC) 方法与质谱 (MS) 相结合对大麻素进行鉴定和定量。我们改进了一种 GC-MS 方法,用于对从不同大麻品种的干花中提取的大麻素进行化学分析。该方法允许同时量化主要大麻素,即大麻二酚 (CBD)、大麻二酚 (CBC)、四氢大麻酚 (THC)、大麻酚 (CBG) 和大麻酚 (CBN)。我们发现名为Cannabis 5-CW的大麻品种的 CBD 含量最高。我们使用改进的 GC-MS 方法对从多种植物中提取的大麻素进行化学分析。大麻样品,尤其是高 CBD、CBC、THC、CBG 和 CBN 生产的大麻样品。该方法在短时间内成功应用于大麻素的鉴定和定量,在已报道的方法中具有更好的分离分辨率。

更新日期:2021-06-28
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