Discovering and screening for potential anti-obesity, anti-diabetic, anticancer, and antioxidant treatments from natural products still in recent times the main goal for many pharmaceutical scientists. The present investigation aimed to evaluate the chemical constituents of Ephedra alata fruits various extracts and to assess their antioxidant, antiobesity, antidiabetic, and cytotoxic effects. In this work, high content of flavonoids and phenols were observed in the methanol fraction of E. alata fruits, which reached 98.95 mg of RUE/g and 33.22 mg of GAE/g, respectively. The methanol fraction has significant inhibitory activity against DPPH, α-amylase, α-glucosidase, and lipase with an IC50 value of 1.07, 9.43, 46.16, and 66.48 µg/mL. respectively. Also has anticancer activity against HeLa cancer cell line. While the acetone fraction has potent antioxidant activity with IC50 5 µg/mL. The DPPH and digestive enzymes assays results showed that the methanolic fraction of E. alata fruits has potent antioxidant, anti-diabetic, and anti-obesity activities, which can be an excellent candidate for biological and chemical analysis and can be further subjected for isolation of the therapeutically active compounds with anticancer potency.

中文翻译：

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麻黄果提取物：植物化学筛选、抗增殖活性和抑制 DPPH、α-淀粉酶、α-葡萄糖苷酶和脂肪酶

从天然产物中发现和筛选潜在的抗肥胖、抗糖尿病、抗癌和抗氧化治疗方法仍然是近代许多药物科学家的主要目标。本研究旨在评估麻黄果实各种提取物的化学成分，并评估它们的抗氧化、抗肥胖、抗糖尿病和细胞毒性作用。在这项工作中，在 E. alata 果实的甲醇部分中观察到高含量的黄酮类化合物和酚类，分别达到 98.95 mg RUE/g 和 33.22 mg GAE/g。甲醇组分对 DPPH、α-淀粉酶、α-葡萄糖苷酶和脂肪酶具有显着的抑制活性，IC50 值为 1.07、9.43、46.16 和 66.48 µg/mL。分别。还具有针对 HeLa 癌细胞系的抗癌活性。而丙酮组分具有有效的抗氧化活性，IC50 为 5 µg/mL。DPPH 和消化酶测定结果表明，E. alata 果实的甲醇组分具有很强的抗氧化、抗糖尿病和抗肥胖活性，是生物和化学分析的极好候选物，可进一步用于分离具有抗癌效力的治疗活性化合物。