Pampa cytoplasmic male sterility phenomenon is used extensively in the rye hybrid breeding programs. It relies on sterilizing action of the cytoplasm resulting in non-viable pollen of female lines. The sterilizing effect is problematic for reversion, and efficient restores are needed. The most promising QTL is located on chromosome 4R, but other chromosomes may also code the trait. Advanced recombinant inbred lines formed bi-parental mapping population genotyped with DArTseq markers. Genetic mapping allowed the seven linkage groups to construct with numerous markers and represent all rye chromosomes. Single marker analysis and composite interval mapping were conducted to identify markers linked to the pollen fertility. Association mapping was used to detect additional markers associated with the trait. A highly significant QTL (QRfp-4R) that explained 42.3% of the phenotypic variation was mapped to the distal part of the long arm of the 4R chromosome. The markers localized in the QRfp-4R region achieve R² association values up to 0.59. The homology of the 43 marker sequences to the loci responsible for fertility restoration in other species and transcription termination factor (mTERF) linked to Rf genes was established. Ten markers were successfully converted into PCR-specific conditions, and their segregation pattern was identical to that of unconverted DArTs.

潘帕细胞质雄性不育现象广泛应用于黑麦杂交育种计划中。它依赖于细胞质的灭菌作用，导致雌性品系无法存活的花粉。杀菌效果对于恢复来说是有问题的，需要高效的恢复。最有希望的 QTL 位于 4R 染色体上，但其他染色体也可能编码该性状。先进的重组自交系形成了用 DArTseq 标记进行基因分型的双亲作图群体。遗传图谱允许七个连锁群用大量标记构建并代表所有黑麦染色体。进行单标记分析和复合区间作图以确定与花粉育性相关的标记。关联图谱用于检测与性状相关的其他标记。解释 42.3% 表型变异的高度显着 QTL ( QRfp-4R ) 被定位到 4R 染色体长臂的远端部分。位于QRfp-4R区域的标记实现 R ²关联值高达 0.59。建立了 43 个标记序列与其他物种中负责生育力恢复的基因座以及与Rf基因相关的转录终止因子 (mTERF) 的同源性。十个标记已成功转换为 PCR 特异性条件，并且它们的分离模式与未转换的 DArT 相同。