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Morphological and molecular identification of Neopestalotiopsis clavispora causing flower blight on Anthurium andraeanum in Thailand
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2021-06-26 , DOI: 10.1016/j.hpj.2020.10.004
Wilailuck Daengsuwan , Prisana Wonglom , Siwaret Arikit , Anurag Sunpapao

Flower blight on anthurium (Anthurium andraeanum) was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand. The fungal isolate was identified as Neopestalotiopsis clavispora based on the morphology and DNA sequence of the internal transcribed spacer (ITS), translation elongation factor 1-α (tef1-α), and β-tubulin (tub) genes. The phylogenetic tree, based on the combined sequences of ITS, tef1-α, and tub, confirmed this pathogen as N. clavispora. Pathogenicity of the species was confirmed according to Koch's postulate: N. clavispora could infect anthurium. To the best of our knowledge, this is the first report of N. clavispora as a pathogen of anthurium.



中文翻译:

引起泰国红掌花枯萎病的Neopestalotiopsis clavispora的形态学和分子鉴定

2018 年 8 月,在泰国南部宋卡府的一个红掌种植农场观察到了红掌(Anthurium andraeanum)花枯病。真菌分离物鉴定为Neopestalotiopsis clavispora基于所述内转录间隔区的形态和DNA序列(ITS),翻译延伸因子1-α(TEF1-α)和β微管蛋白(T UB)的基因。系统发育树基于 ITS、tef1-αtub的组合序列,确认该病原体为N. clavispora。该物种的致病性根据 Koch 的假设得到证实:N. clavispora可以感染红掌。据我们所知,这是第一次将N. clavispora作为红掌病原体的报告。

更新日期:2021-06-26
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