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Fluorescent labeling of s2T-incorporated DNA and m5s2U-modified RNA
Nucleosides, Nucleotides & Nucleic Acids ( IF 1.1 ) Pub Date : 2021-06-26 , DOI: 10.1080/15257770.2021.1942044
Ping Yu 1 , Honglin Zhou 1 , Yuanyuan Li 1 , Zhifeng Du 1 , Rui Wang 1
Affiliation  

Abstract

We report herein comprehensive investigations of alkylation/sulfur exchange reactions of sulfur-containing substrates including nucleosides such as s2U, m5s2U, s4U, s2A and s2T-incorporated DNA enable by comprehensive screenings of the reagents (2a–2h). It has been proven that iodoacetamide (2a) displays the most promising feasibility toward sulfur-containing substrates including s2T, s2U, m5s2U, s4U and s2A. In sharp contrast, the alkylation process with S-benzyl methanethiosulfonate (BMTS, 2h) displays the best application potential only for s4U. Based on these results, the fluorescent labeling of s2T-incorporated DNA and m5s2U-modified RNA has been achieved.

Supplemental data for this article is available online at https://doi.org/10.1080/15257770.2021.1942044 .



中文翻译:


s2T 掺入 DNA 和 m5s2U 修饰 RNA 的荧光标记


 抽象的


我们在此报告了对含硫底物(包括核苷,如 s 2 U、m 5 s 2 U、s 4 U、s 2 A 和 s 2 T 掺入 DNA)的烷基化/硫交换反应的全面研究,通过全面筛选试剂 ( 2a–2h )。事实证明,碘乙酰胺 ( 2a ) 对含硫底物(包括 s 2 T、s 2 U、m 5 s 2 U、s 4 U 和 s 2 A)表现出最有前景的可行性。 S-苄基甲硫代磺酸盐(BMTS, 2h ) 仅对s 4 U 显示出最佳的应用潜力。基于这些结果,已经实现了s 2 T 掺入的DNA 和m 5 s 2 U 修饰的RNA 的荧光标记。

Supplemental data for this article is available online at https://doi.org/10.1080/15257770.2021.1942044 .

更新日期:2021-08-15
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