Cotton yield is affected by drought stress. We performed widely targeted metabolomics to explore metabolic changes under drought stress in the roots and leaves of two cotton varieties differing in drought tolerance—XLZ8 as sensitive and G95079 as tolerant variety. A total of 537 metabolites were detected at different stages of drought stress. Abscisic acid (ABA) was significantly enriched in all stages of drought stress in both roots and leaves of XLZ8. Amino acids showed enrichment in leaves of G95079 in the early stage of drought stress. Flavonoids were significantly enriched in the leaves of the two varieties in the late stage of drought stress. In roots, nucleotides and their derivatives were highly enriched in XLZ8 in the late stage of drought stress. The biosynthetic pathways of kynurenine and polyamine and glycerophospholipid metabolism may play an important role in cotton drought tolerance by scavenging reactive oxygen species (ROS) and reconstructing cell membranes. Spraying appropriate concentrations of proline, kynurenine, S-adenosylmethionine, glucose and 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine (lyso PC18:1) significantly increased the survival rate of cotton seedlings under drought stress. We speculated that ABA-dependent and ABA-independent metabolic changes may lead to differences in drought tolerance among cotton varieties.

中文翻译：

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广泛靶向代谢组学揭示干旱胁迫下两个棉花品种叶和根的不同代谢变化

棉花产量受到干旱胁迫的影响。我们进行了广泛的靶向代谢组学研究，以探索耐旱性不同的两个棉花品种的根和叶在干旱胁迫下的代谢变化——XLZ8 作为敏感品种，G95079 作为耐受品种。在干旱胁迫的不同阶段共检测到537种代谢物。脱落酸（ABA）在干旱胁迫的各个阶段在XLZ8的根和叶中均显着富集。G95079在干旱胁迫早期叶片中氨基酸含量较高。干旱胁迫后期，两个品种叶片中黄酮类化合物含量显着富集。在干旱胁迫后期，XLZ8在根中高度富集核苷酸及其衍生物。犬尿氨酸和多胺的生物合成途径和甘油磷脂代谢可能通过清除活性氧 (ROS) 和重建细胞膜在棉花抗旱性中发挥重要作用。喷洒适当浓度的脯氨酸、犬尿氨酸、S-腺苷甲硫氨酸、葡萄糖和1-油酰-2-羟基-sn-甘油-3-磷酸胆碱（lyso PC18:1）可显着提高干旱胁迫下棉花幼苗的成活率。我们推测ABA依赖和ABA非依赖的代谢变化可能导致棉花品种之间耐旱性的差异。葡萄糖和 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine (lyso PC18:1) 显着提高干旱胁迫下棉花幼苗的存活率。我们推测ABA依赖和ABA非依赖的代谢变化可能导致棉花品种之间耐旱性的差异。葡萄糖和 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine (lyso PC18:1) 显着提高干旱胁迫下棉花幼苗的存活率。我们推测ABA依赖和ABA非依赖的代谢变化可能导致棉花品种之间耐旱性的差异。