The treatment of spinal cord injury is still a challenge worldwide; there is still no effective method. Our strategy is to devise a macrophage-mediated degradable gelatin coated mesoporous silica nanoparticles, which could carry pirfenidone and realize spatiotemporal control of pirfenidone release in the lesion site. For the in vivo experiment, three groups of SD rats subjected to spinal cord contusion injury were injected with GNS-PFD, PFD or PBS. Spinal cord functions were observed. In vitro, we investigated the expression of inflammatory and anti-inflammatory factors. Spinal cord function and recovery were better in the GSN-PFD and PFD than the control group. In the in vitro study, the MMPs after SCI in lesion site were lower in the experimental group. Moreover, the expression of anti-inflammatory and inflammatory factors showed better in the experimental group. The inflammatory response of the PFD to time and space can be achieved with the loading of macrophage-mediated degradable gelatin coated mesoporous silica nanoparticles.

脊髓损伤的治疗在世界范围内仍然是一个挑战；仍然没有有效的方法。我们的策略是设计一种巨噬细胞介导的可降解明胶包覆的介孔二氧化硅纳米粒子，它可以携带吡非尼酮并实现对病变部位吡非尼酮释放的时空控制。对于体内实验，三组脊髓挫伤的SD大鼠分别注射GNS-PFD、PFD或PBS。观察脊髓功能。在体外，我们研究了炎症和抗炎因子的表达。GSN-PFD和PFD组的脊髓功能和恢复情况优于对照组。在体外研究中，实验组损伤部位SCI后的MMPs较低。而且，实验组抗炎和炎症因子表达较好。PFD对时间和空间的炎症反应可以通过加载巨噬细胞介导的可降解明胶涂层介孔二氧化硅纳米粒子来实现。