Abstract In this study, optimal conditions to form cellulose-MgO nanocomposite with antibacterial properties were evaluated. Applying the Taguchi method, 9 experiments were designed and the effects of different concentrations of biopolymers cellulose (0.5, 1 and 2 mg/ml), MgO nanoparticles (2, 4 and 8 mg/ml) and stirring times (30, 60 and 90 min) on antibacterial activity of synthesized nanocomposites were assessed. The characterizations of products were investigated by dynamic light scattering (DLS), raman spectroscopy, scanning electron microscope (SEM), thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The results showed that the nano-composite produced in the conditions of experiment 9 (MgO 8 mg/ml, cellulose 2 mg/ml and stirring time of 60 min) has the strongest antibacterial activity. The outcomes of both methods of colony forming units (CFU) and disc diffusion indicated that the antibacterial activity of cellulose-MgO nanocomposite was significantly higher than its components (P <0.05). Thermal analysis indicated improvement in the thermal stability of the cellulose biopolymer after the formation of the nanocomposite. Due to the improvement of the antibacterial properties of cellulose-MgO nanocomposite compared to its components, we can use it as a new antibacterial agent in the fields of pharmaceutical, medicine and dentistry.

中文翻译：

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田口法在抗菌剂纤维素-氧化镁生物纳米复合材料合成优化中的应用

摘要 在这项研究中，评估了形成具有抗菌性能的纤维素-氧化镁纳米复合材料的最佳条件。应用田口方法，设计了 9 个实验，不同浓度的生物聚合物纤维素（0.5、1 和 2 毫克/毫升）、氧化镁纳米粒子（2、4 和 8 毫克/毫升）和搅拌时间（30、60 和 90 min) 对合成纳米复合材料的抗菌活性进行了评估。通过动态光散射 (DLS)、拉曼光谱、扫描电子显微镜 (SEM)、热重分析 (TGA) 和差热分析 (DTA) 研究了产物的表征。结果表明，实验9条件下（MgO 8 mg/ml，纤维素2 mg/ml，搅拌时间60 min）制备的纳米复合材料抗菌活性最强。菌落形成单位（CFU）和圆盘扩散两种方法的结果表明，纤维素-MgO纳米复合材料的抗菌活性显着高于其组分（P <0.05）。热分析表明，在形成纳米复合材料后，纤维素生物聚合物的热稳定性有所改善。由于纤维素-氧化镁纳米复合材料的抗菌性能与其组分相比有所提高，我们可以将其用作制药、医学和牙科领域的新型抗菌剂。热分析表明，在形成纳米复合材料后，纤维素生物聚合物的热稳定性有所改善。由于纤维素-氧化镁纳米复合材料的抗菌性能与其组分相比有所提高，我们可以将其用作制药、医学和牙科领域的新型抗菌剂。热分析表明，在形成纳米复合材料后，纤维素生物聚合物的热稳定性有所改善。由于纤维素-氧化镁纳米复合材料的抗菌性能与其组分相比有所提高，我们可以将其用作制药、医学和牙科领域的新型抗菌剂。