The objective of this study was to find out if suppression of NF-kB complex function by p65-TMD-linked PTD could reduce host inflammation and bone resorption at peri-implantitis sites in rats. Twenty-one male 5-week-old SD rats were divided into three groups: untreated control group (A), silk-induced peri-implantitis group (B), and nt (nucleus transducible)-p65-TMD-treated, silk-induced peri-implantitis group (C). Implant sulcus of a rat in group C were divided into two groups, namely group Cp and Cb. Palatal implant sulcus where nt-p65-TMD solution was applied with an insulin syringe were assigned to group Cp. Buccal implant sulcus without topical nt-p65-TMD application were assigned to group Cb. H&E staining, TRAP staining, and immunohistological staining were done. The crestal bone levels of group A were significantly higher than those of group B at p<0.01. The crestal bone levels of group Cp were significantly higher than those of group Cb at p<0.05. H-E staining showed increased apical migration of junctional epithelium and inflammatory cells in group Cb. TRAP staining revealed more multinucleated osteoclasts in group Cb. As for immunohistological staining, group Cb showed many IL-6-positive cells while group Cp had none. In this study, p65-TMD-linked PTD inhibited NF-kB functions and reduced inflammation and bone resorption at peri-implantitis sites in rats.

本研究的目的是确定 p65-TMD 连接的 PTD 抑制 NF-kB 复合物功能是否可以减少大鼠种植体周围炎部位的宿主炎症和骨吸收。将 21 只雄性 5 周龄 SD 大鼠分为三组：未处理的对照组 (A)、丝诱导的种植体周围炎组 (B) 和 nt（核转导）-p65-TMD 处理的、丝-诱导种植体周围炎组（C）。C组大鼠种植体沟分为两组，即Cp组和Cb组。用胰岛素注射器应用nt-p65-TMD溶液的腭种植体沟被分配到Cp组。没有局部 nt-p65-TMD 应用的颊侧种植体沟被分配到 Cb 组。进行H&E染色、TRAP染色和免疫组织学染色。A组嵴骨水平显着高于B组，p<0.01。Cp组嵴骨水平显着高于Cb组，p<0.05。HE染色显示Cb组交界上皮和炎性细胞的顶端迁移增加。TRAP 染色显示 Cb 组中有更多的多核破骨细胞。免疫组化染色，Cb组IL-6阳性细胞较多，Cp组无。在这项研究中，p65-TMD 连接的 PTD 可抑制大鼠种植体周围炎部位的 NF-kB 功能并减少炎症和骨吸收。TRAP 染色显示 Cb 组中有更多的多核破骨细胞。免疫组化染色，Cb组IL-6阳性细胞较多，Cp组无。在这项研究中，p65-TMD 连接的 PTD 可抑制大鼠种植体周围炎部位的 NF-kB 功能并减少炎症和骨吸收。TRAP 染色显示 Cb 组中有更多的多核破骨细胞。免疫组化染色，Cb组IL-6阳性细胞较多，Cp组无。在这项研究中，p65-TMD 连接的 PTD 可抑制大鼠种植体周围炎部位的 NF-kB 功能并减少炎症和骨吸收。