Progress in NMR in general and in biomolecular applications in particular is driven by increasing magnetic-field strengths leading to improved resolution and sensitivity of the NMR spectra. Recently, persistent superconducting magnets at a magnetic field strength (magnetic induction) of 28.2 T corresponding to 1200 MHz proton resonance frequency became commercially available. We present here a collection of high-field NMR spectra of a variety of proteins, including molecular machines, membrane proteins, viral capsids, fibrils and large molecular assemblies. We show this large panel in order to provide an overview over a range of representative systems under study, rather than a single best performing model system. We discuss both carbon-13 and proton-detected experiments, and show that in ¹³C spectra substantially higher numbers of peaks can be resolved compared to 850 MHz while for ¹H spectra the most impressive increase in resolution is observed for aliphatic side-chain resonances.

核磁共振的进展，特别是在生物分子应用方面的进展，是由不断增加的磁场强度推动的，从而提高了核磁共振谱的分辨率和灵敏度。最近，对应于 1200 MHz 质子共振频率的磁场强度（磁感应强度）为 28.2 T 的持久性超导磁体开始商用。我们在此展示了各种蛋白质的高场 NMR 谱图，包括分子机器、膜蛋白、病毒衣壳、原纤维和大分子组装体。我们展示这个大面板是为了提供对正在研究的一系列代表性系统的概述，而不是单个最佳性能模型系统。我们讨论了碳 13 和质子检测实验，并表明在¹³与 850 MHz 相比，C 光谱可以分辨出更多数量的峰，而对于¹ H 光谱，脂肪族侧链共振的分辨率提高最为显着。