Introduction. Colistin is one of the last-resort antibiotics for treating multidrug-resistant (MDR) or extensively drug-resistant (XDR) lactose non-fermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii . Gap Statement. As the rate of colistin resistance is steadily rising, there is a need for rapid and accurate antimicrobial susceptibility testing methods for colistin. The Rapid ResaPolymyxin Acinetobacter / Pseudomonas NP test has recently been developed for rapid detection of colistin resistance in P. aeruginosa and A. baumannii . Aim. The present study aimed to evaluate the performance of the Rapid ResaPolymyxin Acinetobacter / Pseudomonas NP test in comparison with the reference broth microdilution (BMD) method. Methodology. The Rapid ResaPolymyxin Acinetobacter / Pseudomonas NP test was performed using a total of 135 P . aeruginosa (17 colistin-resistant and 118 colistin-susceptible) and 66 A. baumannii isolates (32 colistin-resistant and 34 colistin-susceptible), in comparison with the reference BMD method. Results. The categorical agreement of the Rapid ResaPolymyxin Acinetobacter / Pseudomonas NP test with the reference BMD method was 97.5 % with a major error rate of 0 % (0/152) and a very major error (VME) rate of 10.2 %. The VME rate was higher (23.5 %) when calculated separately for P. aeruginosa isolates. The overall sensitivity and specificity were 89.8 and 100 %, respectively. Conclusion. The Rapid ResaPolymyxin Acinetobacter / Pseudomonas NP test performed better for A. baumannii than for P. aeruginosa .

中文翻译：

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评估 ResaPolymyxin Acinetobacter/Pseudomonas NP 快速检测对乳糖非发酵革兰氏阴性菌中粘菌素耐药性的快速检测

介绍。粘菌素是治疗耐多药 (MDR) 或广泛耐药 (XDR) 乳糖非发酵革兰氏阴性菌（如铜绿假单胞菌和鲍曼不动杆菌）的最后选择抗生素之一。差距声明。随着粘菌素耐药率稳步上升，需要对粘菌素进行快速准确的抗菌药敏试验。最近开发了快速 ResaPolymyxin Acinetobacter / Pseudomonas NP 测试，用于快速检测铜绿假单胞菌和鲍曼不动杆菌中的粘菌素耐药性。目标。 本研究旨在评估快速 ResaPolymyxin Acinetobacter / Pseudomonas NP 测试与参考肉汤微量稀释 (BMD) 方法的性能。方法。快速 ResaPolymyxin Acinetobacter / Pseudomonas NP 测试使用总共 135 P 进行。与参考 BMD 方法相比，铜绿假单胞菌（17 个粘菌素抗性和 118 个粘菌素敏感）和 66 个鲍曼不动杆菌分离株（32 个粘菌素抗性和 34 个粘菌素敏感）。结果。Rapid ResaPolymyxin Acinetobacter的绝对一致性/ 使用参考 BMD 方法进行的假单胞菌NP 测试为 97.5%，主要错误率为 0% (0/152)，非常主要错误 (VME) 率为 10.2%。当单独计算铜绿假单胞菌分离株时，VME 率更高（23.5%）。总体敏感性和特异性分别为 89.8% 和 100%。结论。快速 ResaPolymyxin Acinetobacter / Pseudomonas NP 测试对鲍曼不动杆菌比对铜绿假单胞菌表现更好。