Surface morphology of implants plays an important role in enhancing cell response at the interface. Zinc ions (Zn²⁺) have a promising potential for osseointegration by stimulating osteoblast cell multiplication and differentiation. In this study, regular micro grooves coated with zinc oxide (ZnO) were successfully manufactured on Ti-6Al-4V surface by two-step nanosecond laser treatment to investigate the synergetic effects of micro/nano structures and Zn²⁺ on the cell adhesion, proliferation and differentiation. Surface characteristics including surface morphology, roughness, wettability and phase composition were characterized. Results showed that the roughness and hydrophilicity of laser-processing surface were significantly improved compared with those of the acid etching surface. Moreover, XRD results indicated that the ZnO diffraction peak appeared on the surface. The biocompatibility of different samples was investigated by cell experiment in vitro. The spreading morphology of cells along grooves, cell multiplication rate, and activity of alkaline phosphatase and osteocalcin indicated that the micro grooves coated with ZnO on Ti-6Al-4V surface had great potential to promote the adhesion, multiplication and differentiation of MC3T3 cells. This study provides a new and effective method for surface modification of Ti-6Al-4V implants.

植入物的表面形态在增强界面处的细胞反应方面起着重要作用。锌离子 (Zn ²⁺ ) 通过刺激成骨细胞增殖和分化而具有良好的骨整合潜力。在这项研究中，通过两步纳秒激光处理在 Ti-6Al-4V 表面成功制造了氧化锌 (ZnO) 涂层的规则微凹槽，以研究微/纳米结构与 Zn ²⁺的协同效应对细胞粘附、增殖和分化的影响。表征了表面特征，包括表面形态、粗糙度、润湿性和相组成。结果表明，与酸蚀表面相比，激光加工表面的粗糙度和亲水性显着提高。此外，XRD 结果表明 ZnO 衍射峰出现在表面。通过体外细胞实验研究不同样品的生物相容性。细胞沿沟的铺展形态、细胞增殖率、碱性磷酸酶和骨钙素的活性表明，Ti-6Al-4V表面包覆ZnO的微沟具有促进MC3T3细胞粘附、增殖和分化的潜力。