Micropipette is a quite simple, but an essential instrument used for the volume measurements and finds the applications in the field of chemistry, pharmacy, health care, biology, pathology, research and development, etc. The calibration laboratories must ensure that the results obtained using these instruments are accurate, and therefore, the calibration is an inevitable process. This report is on the micropipette calibration techniques with different methods considering various influencing factors those affect the measurement results. The measurements were done in controlled environmental conditions and taking all the necessary precautions. This report also presents a detailed comparison between the three distinct test methods used, i.e. “conventional method”, “with evaporation loss correction” method and “without evaporation loss correction” method. This report summarizes how the change of techniques applied can affect the final volume of the micropipette and the associated uncertainty in the measurement. This study will contribute to the improvement of the whole process of micropipette calibration for performing the calibration in volume. Further such studies can also be conducted for glassware calibration to improve the overall upgradation of volume calibration.

微量移液器是一种非常简单但必不可少的用于体积测量的仪器，在化学、药学、医疗保健、生物学、病理学、研究和开发等领域都有应用。校准实验室必须确保使用获得的结果这些仪器是准确的，因此校准是一个不可避免的过程。本报告是关于采用不同方法的微量移液器校准技术，考虑了影响测量结果的各种影响因素。测量是在受控的环境条件下进行的，并采取了所有必要的预防措施。该报告还详细比较了所使用的三种不同测试方法，即“常规方法”、“有蒸发损失修正”法和“无蒸发损失修正”法。本报告总结了应用技术的变化如何影响微量移液器的最终体积以及测量中的相关不确定性。该研究将有助于改进微量移液器校准以进行体积校准的整个过程。进一步的此类研究也可以用于玻璃器皿校准，以改善体积校准的整体升级。