Nitrate uptake has attracted more and more attention by far. It is known that sucrose is the signal molecule of nitrate uptake by wheat roots, which starts to increase immediately after sucrose addition. To make sure the action of sucrose signal, the effects of 10 mM and 1 mM sucrose on nitrate uptake in the light/dark were compared. Considering the intact plant was necessary to investigate the mechanisms involved in metabolism, a non-invasive technique had been used. After sucrose addition, net nitrate uptake kept increasing. It was known that sucrose effect in the dark was more effective than in the light. We found that as compared to the control, the increment of net nitrate uptake in the light was 3.8–7.3 times after sucrose addition, the effect of different sucrose concentrations was not significantly different. After sucrose removal, there was no significant difference in the lag before the response of nitrate uptake and recovery time to the original level under different light conditions (light/dark), but there was a significant difference between high and low concentrations. For halftime of the response after removal, they were significantly different between different light conditions and sucrose concentrations. The detailed dynamics analysis of net nitrate uptake by roots was analyzed and discussed after sucrose addition and removal. The work provided data support and referential value for improving N use efficiency, which was therefore important to investigate N metabolism and decrease the waste of N fertilizer.

到目前为止，硝酸盐的吸收已经引起了越来越多的关注。众所周知，蔗糖是小麦根系吸收硝酸盐的信号分子，加入蔗糖后立即开始增加。为了确定蔗糖信号的作用，比较了 10 mM 和 1 mM 蔗糖对明/暗中硝酸盐吸收的影响。考虑到完整的植物是研究代谢机制所必需的，因此使用了一种非侵入性技术。添加蔗糖后，硝酸盐的净吸收量不断增加。众所周知，蔗糖在黑暗中的作用比在光下更有效。我们发现，与对照相比，添加蔗糖后，光照下硝酸盐的净吸收增量为3.8-7.3倍，不同蔗糖浓度的影响无显着差异。去除蔗糖后，不同光照条件（明/暗）下硝酸盐吸收和恢复时间响应至初始水平前的滞后时间无显着差异，但高、低浓度之间存在显着差异。对于去除后的半场响应，它们在不同光照条件和蔗糖浓度之间存在显着差异。对添加和去除蔗糖后根系净硝酸盐吸收的详细动力学分析进行了分析和讨论。该工作为提高氮素利用效率提供了数据支持和参考价值，对研究氮素代谢和减少氮肥浪费具有重要意义。但高浓度和低浓度之间存在显着差异。对于去除后的半场响应，它们在不同光照条件和蔗糖浓度之间存在显着差异。对添加和去除蔗糖后根系净硝酸盐吸收的详细动力学分析进行了分析和讨论。该工作为提高氮素利用效率提供了数据支持和参考价值，对研究氮素代谢和减少氮肥浪费具有重要意义。但高浓度和低浓度之间存在显着差异。对于去除后的半场响应，它们在不同光照条件和蔗糖浓度之间存在显着差异。对添加和去除蔗糖后根系净硝酸盐吸收的详细动力学分析进行了分析和讨论。该工作为提高氮素利用效率提供了数据支持和参考价值，对研究氮素代谢和减少氮肥浪费具有重要意义。对添加和去除蔗糖后根系净硝酸盐吸收的详细动力学分析进行了分析和讨论。该工作为提高氮素利用效率提供了数据支持和参考价值，对研究氮素代谢和减少氮肥浪费具有重要意义。对添加和去除蔗糖后根系净硝酸盐吸收的详细动力学分析进行了分析和讨论。该工作为提高氮素利用效率提供了数据支持和参考价值，对研究氮素代谢和减少氮肥浪费具有重要意义。