Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.

艰难梭菌BI/NAP1/ribotype 027 是一种流行的超毒株，在世界范围内发现，包括在拉丁美洲。我们检查了两种多位点序列类型 (MLST) 进化枝 2艰难梭菌菌株的基因组和外蛋白质组，它们被认为具有高毒力：ICC-45（核糖型 SLO231/UK[CE]821），在巴西分离，和 NAP1/027/ST01 (LIBA5756) ，在 2010 年哥斯达黎加爆发期间被隔离。艰难梭菌培养分离株并使用高效液相色谱-串联质谱法分析细胞外蛋白。基因组分析表明，这些分离株共享大部分基因组成。只有 83 和 290 NAP1/027 基因分别被认为是 ICC-45 和 NAP1/027 中的单例。外蛋白质组分析揭示了 197 种蛋白质，其中 192 种在两种菌株中相似。只有五种蛋白质是 ICC-45 菌株独有的。这些蛋白质与催化和结合功能有关，并与与致病性相关的蛋白质间接相互作用。大多数蛋白质，包括 TcdA、TcdB、鞭毛蛋白亚基和细胞表面蛋白，在 ICC-45 菌株中含量过高；LIBA5756 中有 14 种蛋白质，包括成熟的 S 层蛋白质，以更高的比例存在。数据可通过 ProteomeXchange 获得，标识符为 PXD026218。这些数据表明，在拉丁美洲分离出的具有高毒力特征的两种菌株上清液中的基因组和蛋白质之间存在密切的相似性，并强调了对新菌株的传播和出现进行流行病学监测的重要性。