Layered molybdenum diselenide (MoSe₂) nanosheets were formed by the weak Van der Waals forces of attraction between Se and Mo atoms. MoSe₂ has a larger space between the adjacent layers and smaller band gaps in the range of 0.85 to ~ 1.6 eV. In this study, MoSe₂ nanosheets decorated nickel oxide (NiO) nanorods have been synthesized by hydrothermal method using sodium molybdate and selenium metal powder. NiO/MoSe₂ composite formation was confirmed by powder X-ray diffraction analysis. In addition, the presence of MoSe₂ nanosheets on NiO nanorods were confirmed by field emission scanning electron microscopy, high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The Nyquist plots of NiO/MoSe₂ coated glassy carbon electrode (GCE) was indicated that it had lower charge transfer resistance compared to NiO/GCE and MoSe₂/GCE. Furthermore, as-prepared NiO/MoSe₂/GCE was used to detect glucose in alkaline solution by cyclic voltammetry and amperometry techniques. The NiO/MoSe₂/GCE was exhibited a linear response for the oxidation of glucose from 50 µM to 15.5 mM (R² = 0.9842) at 0.5 V by amperometry. The sensor response time and the limit of detection were found to be 2 s and 0.6 µM for glucose. Moreover, selectivity of the NiO/MoSe₂ sensor was tested in the presence of common interferent molecules such as hydrogen peroxide, fructose, lactose, ascorbic acid, uric acid, and dopamine. It was found that NiO/MoSe₂/GCE did not respond to these interfering biomolecules. In addition, NiO/MoSe₂/GCE had shown high stability, reproducibility and repeatability. Finally, the practical application of the sensor was demonstrated by detecting glucose in human blood serum with the acceptable recovery.

层状二硒化钼 (MoSe ₂ ) 纳米片由 Se 和 Mo 原子之间的弱范德华力形成。MoSe ₂在相邻层之间具有更大的空间，并且在 0.85 至 ~ 1.6 eV 的范围内具有更小的带隙。在这项研究中，使用钼酸钠和硒金属粉末通过水热法合成了MoSe ₂纳米片装饰的氧化镍 (NiO) 纳米棒。NiO/MoSe ₂复合物的形成由粉末X射线衍射分析证实。此外，MoSe ₂的存在通过场发射扫描电子显微镜、高分辨率透射电子显微镜和 X 射线光电子能谱证实了 NiO 纳米棒上的纳米片。NiO/MoSe ₂涂层玻碳电极(GCE)的奈奎斯特图表明，与NiO/GCE 和MoSe ₂ /GCE相比，它具有较低的电荷转移电阻。此外，制备的 NiO/MoSe ₂ /GCE 用于通过循环伏安法和电流法技术检测碱性溶液中的葡萄糖。NiO/MoSe ₂ /GCE 对葡萄糖从 50 µM 氧化到 15.5 mM (R ² = 0.9842) 在 0.5 V 时通过电流分析法。发现传感器响应时间和检测限为 2 秒，葡萄糖为 0.6 µM。此外，在常见干扰分子如过氧化氢、果糖、乳糖、抗坏血酸、尿酸和多巴胺存在的情况下，测试了 NiO/MoSe ₂传感器的选择性。发现NiO/MoSe ₂ /GCE 对这些干扰生物分子没有反应。此外，NiO/MoSe ₂ /GCE 已显示出高稳定性、再现性和可重复性。最后，通过以可接受的回收率检测人血清中的葡萄糖来证明传感器的实际应用。