Layered molybdenum diselenide (MoSe₂) nanosheets were formed by the weak Van der Waals forces of attraction between Se and Mo atoms. MoSe₂ has a larger space between the adjacent layers and smaller band gaps in the range of 0.85 to ~ 1.6 eV. In this study, MoSe₂ nanosheets decorated nickel oxide (NiO) nanorods have been synthesized by hydrothermal method using sodium molybdate and selenium metal powder. NiO/MoSe₂ composite formation was confirmed by powder X-ray diffraction analysis. In addition, the presence of MoSe₂ nanosheets on NiO nanorods were confirmed by field emission scanning electron microscopy, high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The Nyquist plots of NiO/MoSe₂ coated glassy carbon electrode (GCE) was indicated that it had lower charge transfer resistance compared to NiO/GCE and MoSe₂/GCE. Furthermore, as-prepared NiO/MoSe₂/GCE was used to detect glucose in alkaline solution by cyclic voltammetry and amperometry techniques. The NiO/MoSe₂/GCE was exhibited a linear response for the oxidation of glucose from 50 µM to 15.5 mM (R² = 0.9842) at 0.5 V by amperometry. The sensor response time and the limit of detection were found to be 2 s and 0.6 µM for glucose. Moreover, selectivity of the NiO/MoSe₂ sensor was tested in the presence of common interferent molecules such as hydrogen peroxide, fructose, lactose, ascorbic acid, uric acid, and dopamine. It was found that NiO/MoSe₂/GCE did not respond to these interfering biomolecules. In addition, NiO/MoSe₂/GCE had shown high stability, reproducibility and repeatability. Finally, the practical application of the sensor was demonstrated by detecting glucose in human blood serum with the acceptable recovery.

层状二硒化钼（MoSe ₂ ）纳米片是由 Se 和 Mo 原子之间的弱范德华力形成的。 MoSe ₂在相邻层之间具有较大的空间和在0.85至约1.6eV范围内的较小带隙。在这项研究中，使用钼酸钠和硒金属粉末通过水热法合成了MoSe ₂纳米片装饰的氧化镍（NiO）纳米棒。通过粉末X射线衍射分析证实了NiO/MoSe ₂复合材料的形成。此外，通过场发射扫描电子显微镜、高分辨率透射电子显微镜和X射线光电子能谱证实了NiO纳米棒上MoSe ₂纳米片的存在。 NiO/MoSe ₂涂层玻碳电极(GCE)的奈奎斯特图表明，与NiO/GCE和MoSe ₂ /GCE相比，其具有较低的电荷转移电阻。此外，使用所制备的NiO/MoSe ₂ /GCE通过循环伏安法和安培法技术检测碱性溶液中的葡萄糖。通过电流分析法，NiO/MoSe ₂ /GCE 在 0.5 V 电压下对葡萄糖氧化从 50 µM 到 15.5 mM (R ² = 0.9842) 表现出线性响应。发现葡萄糖的传感器响应时间和检测限为 2 秒和 0.6 µM。此外，在常见干扰分子如过氧化氢、果糖、乳糖、抗坏血酸、尿酸和多巴胺存在下测试了NiO/MoSe ₂传感器的选择性。结果发现NiO/MoSe ₂ /GCE对这些干扰生物分子没有反应。 此外，NiO/MoSe ₂ /GCE表现出较高的稳定性、重现性和重复性。最后，通过检测人体血清中的葡萄糖，证明了该传感器的实际应用，并取得了良好的回收率。