The current study is aimed at preparing light-driven novel functional AgNPs- bio-hydrogel and evaluating anticancer potency against human melanoma cells. With an average size of 16–18 nm, the hydrogel nano-silver particle composite (AgNPs@C_MA_O) was synthesized using a soft white LED approach and analyzed by UV–Vis, DLS, FTIR, X-ray, SEM–EDX and TEM techniques. The anticancer activity of the obtained novel functionalized AgNPs@C_MA_O was tested in-vitro in the A375 melanoma cell line. Dose–response analysis showed that AgNPs at 0.01 mg/mL and 0.005 mg/mL doses reduced the viability of A375 cells by 50% at 24 and 48-h time-points, respectively. A375 cells treated with AgNPs@C_MA_O for 24 h at IC50 displayed abnormal morphology such as detachment edges and feet, shrinkage, membrane damage, and the loss of contact with adjacent cells. Our work is the first study showing that non-ionizing radiation mediated biofunctionalized AgNPs have an anti-tumoral effect at such a low concentration of 0.01 mg/mL. Our approach of using harmless wLED increased synergy between soft biopolymer compounds and AgNPs, and enhanced anticancer efficiency of the AgNPs@C_MA_O biohydrogel. Ultimately, the AgNPs accessed through the use of the wLED approach in colloidal syntheses can open new applications and combinatorial advanced cancer treatments and diagnostics.

目前的研究旨在制备光驱动的新型功能性AgNPs-生物水凝胶并评估对人类黑色素瘤细胞的抗癌效力。采用软白光 LED 方法合成平均尺寸为 16-18 nm 的水凝胶纳米银颗粒复合材料 (AgNPs@C_MA_O)，并通过 UV-Vis、DLS、FTIR、X 射线、SEM-EDX 和 TEM 进行分析技术。所获得的新型功能化 AgNPs@C_MA_O 的抗癌活性在 A375 黑色素瘤细胞系中进行了体外测试。剂量反应分析表明，0.01 mg/mL 和 0.005 mg/mL 剂量的 AgNP 在 24 小时和 48 小时时间点分别使 A375 细胞的活力降低 50%。用 AgNPs@C_MA_O 以 IC50 处理 24 小时的 A375 细胞表现出异常形态，如边缘和足部脱离、收缩、膜损伤以及与相邻细胞失去接触。我们的工作是第一项研究表明非电离辐射介导的生物功能化 AgNP 在 0.01 mg/mL 的低浓度下具有抗肿瘤作用。我们使用无害的 wLED 的方法增加了软生物聚合物化合物和 AgNP 之间的协同作用，并提高了 AgNPs@C_MA_O 生物水凝胶的抗癌效率。最终，通过在胶体合成中使用 wLED 方法获得的 AgNP 可以开辟新的应用以及组合先进的癌症治疗和诊断。