ABSTRACT

In the present work, we have attempted to investigate influence of nanocarrier composition and structure on the RBC membrane perturbations which delivers nanocarrier contents inside the cell. For this purpose, blank liposomes (LIP), lipid nanoparticles (NAN) and cubosomes (CUB) were prepared using ethanol injection method. The interaction of nanocarriers with RBC membrane was investigated using confocal microscopy and small angle x-ray scattering (SAXS). ‘d’ value obtained from SAXS data was plotted against time to investigate rate of fusion of nanocarriers with RBC membrane. Amongst all the nanocarriers tested, NAN and CUB were found to be superior over conventional LIP in terms of fusion with RBC membrane. Additionally, the study showed that use of Glyceryl monooleate in the preparation of nanocarriers will help in faster drug/gene delivery inside the RBCs. Thus NAN and CUB can act as better nanocarriers for guest molecules when such molecules are to be delivered inside RBCs.

摘要

在目前的工作中，我们试图研究纳米载体的组成和结构对红细胞膜扰动的影响，红细胞膜在细胞内传递纳米载体内容物。为此，使用乙醇注射法制备空白脂质体（LIP）、脂质纳米颗粒（NAN）和立方体（CUB）。使用共聚焦显微镜和小角 X 射线散射 (SAXS) 研究了纳米载体与 RBC 膜的相互作用。从 SAXS 数据获得的“d”值与时间作图，以研究纳米载体与 RBC 膜的融合率。在所有测试的纳米载体中，发现 NAN 和 CUB 在与 RBC 膜的融合方面优于传统 LIP。此外，该研究表明，在制备纳米载体时使用单油酸甘油酯将有助于更快地在红细胞内传递药物/基因。因此，当客体分子要在红细胞内传递时，NAN 和 CUB 可以作为更好的纳米载体。